Print Email Facebook Twitter Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori Title Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori Author Lokman, B.C. Joosten, V. Hovenkamp, J. Gouka, R.J. Verrips, C.T. van den Hondel, C.A.M.J.J. Publication year 2003 Abstract The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l-1 in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was produced. In contrast to several other studies about heterologous production of heme containing peroxidases, our results suggest that in A. awamori no heme limitation exists during overproduction of ARP. © 2003 Elsevier Science B.V. All rights reserved. Subject BiotechnologyAspergillus awamoriHeme limitationHeterologous protein productionPeroxidaseCell cultureEnzyme kineticsFungiGrowth kineticsMolecular weightWestern blot analysisBiotechnologyfungal enzymeperoxidasexylan endo 1,3 beta xylosidasearthromyces ramosusarticleAspergillus awamoriculture mediumenzyme analysisenzyme synthesisfungusmolecular weightnonhumanpriority journalWestern blottingAspergillusBiotechnologyBlotting, NorthernBlotting, SouthernFermentationHemeMitosporic FungiPeroxidasePlasmidsPromoter Regions (Genetics)'Arthromyces ramosus'AspergillusAspergillus awamoriFungi To reference this document use: http://resolver.tudelft.nl/uuid:34159064-48be-4ce7-ae2c-58a97a277b68 DOI https://doi.org/10.1016/s0168-1656(03)00109-3 TNO identifier 237151 ISSN 0168-1656 Source Journal of Biotechnology, 103 (2), 183-190 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.