Determination of the B2 vitamer flavin-adenine dinucleotide in whole blood by high-performance liquid chromatography with fluorometric detection

Title

Determination of the B2 vitamer flavin-adenine dinucleotide in whole blood by high-performance liquid chromatography with fluorometric detection

 Author

Speek, A.J.
van Schaik, F.
Schrijver, J.
Schreurs, W.H.P.
Centraal Instituut voor Voedingsonderzoek TNO

Publication year

1982

 Abstract

A reliable high-performance liquid chromatographic (HPLC) method is developed for the analysis of FAD in whole blood of humans. The method is able to separate FAD, FMN and Rb from each other and from interfering compounds. A reliable and sensitive detection of FAD has been obtained by selecting the optimal pH of the mobile phase and optimal adjustment of the fluorescence detector.

 Subject

Nutrition
Flavine adenine nucleotide
Flavine nucleotide
Riboflavin
Nicotinamide adenine dinucleotide
Blood
Blood and hemopoietic system
Drug blood level
Drug determination
Fluorometry
High performance liquid chromatography
Human
Human cell
In vitro study
Methodology
Normal human
Normal value
Preliminary communication
Theoretical study
Article
Chemistry
Spectrofluorometry
Chromatography, High Pressure Liquid
Human
Microchemistry
NAD
Spectrometry, Fluorescence

 To reference this document use:

http://resolver.tudelft.nl/uuid:2964521e-25c5-4737-9091-253fea121b83

 TNO identifier

229274

 Source

Journal of Chromatography, 228, 311-316

 Document type

article
Files
To receive the publication files, please send an e-mail request to TNO Library.