Print Email Facebook Twitter Effect of neutralizing sera on factor X-mediated adenovirus serotype 5 gene transfer Title Effect of neutralizing sera on factor X-mediated adenovirus serotype 5 gene transfer Author Parker, A.L. Waddington, S.N. Buckley, S.M.K. Custers, J. Havenga, M.J.E. van Rooijen, N. Goudsmit, J. McVey, J.H. Nicklin, S.A. Baker, A.H. TNO Kwaliteit van Leven Publication year 2009 Abstract The deployment of adenovirus serotype 5 (Ad5)-based vectors is hampered by preexisting immunity. When such vectors are delivered intravenously, hepatocyte transduction is mediated by the hexon-coagulation factor X (FX) interaction. Here, we demonstrate that human sera efficiently block FX-mediated cellular binding and transduction of Ad5-based vectors in vitro. Neutralizing activity correlated well with the ability to inhibit Ad5-mediated liver transduction, suggesting that prescreening patient sera in this manner accurately predicts the efficacy of Ad5-based gene therapies. Neutralization in vitro can be partially bypassed by pseudotyping with Ad45 fiber protein, indicating that a proportion of neutralizing antibodies are directed against the Ad5 fiber. Copyright © 2009, American Society for Microbiology. All Rights Reserved. DOI Subject BiologyBiomedical ResearchAdenovirusarticlecorrelation analysisfibergene therapygene transfergenetic transductionimmunityin vitro studyliver cellnonhumanpriority journalpseudotypingscreeningserotypegene vectorhumanimmunologymetabolismmethodologyserodiagnosistumor cell linevirologyvirus attachmentAdenoviridaeantiserumblood clotting factor 10AdenoviridaeCell Line, TumorFactor XGene TherapyGenetic VectorsHepatocytesHumansImmune SeraNeutralization TestsTransduction, GeneticVirus Attachment To reference this document use: http://resolver.tudelft.nl/uuid:2414e48c-c404-4661-a153-171365b4391c DOI https://doi.org/10.1128/jvi.01878-08 TNO identifier 285077 Source Journal of Virology, 83 (1), 479-483 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.