Title
Filamentous fungi as cell factories for heterologous protein production
Author
Punt, P.J.
van Biezen, N.
Conesa, A.
Albers, A.
Mangnus, J.
van den Hondel, C.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2002
Abstract
Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools have enabled several new developments. But it has been process development that has driven the final breakthrough to achieving commercially relevant quantities of protein. Recent research into gene expression in filamentous fungi has explored their wealth of genetic diversity with a view to exploiting them as expression hosts and as a source of new genes. Inevitably, the progress in the 'genomics' technology will further develop high-throughput technologies for these organisms. Chemicals/CAS: Interleukin-6; manganese peroxidase, EC 1.11.1.13; Peroxidases, EC 1.11.1.-; Recombinant Proteins
Subject
Nutrition
Fermentation
Fungi
Humans
Interleukin-6
Peroxidases
Protein Conformation
Recombinant Proteins
Ascomycota
Aspergillus
Basidiomycota
Fungi
Phanerochaete
Saccharomyces
Saccharomyces cerevisiae
Zygomycetes
To reference this document use:
http://resolver.tudelft.nl/uuid:223d8673-e8fb-4615-9389-0902074e6719
DOI
https://doi.org/10.1016/s0167-7799(02)01933-9
TNO identifier
87836
ISSN
0167-7799
Source
Trends in Biotechnology, 20 (5), 200-206
Document type
article