Title
Detection of sulfur mustard adducts in human callus by phage antibodies
Author
Bikker, F.J.
Mars-Groenendijk, R.H.
Noort, D.
Fidder, A.
van der Schans, G.P.
TNO Defensie en Veiligheid
Publication year
2007
Abstract
As part of a research program to develop novel methods for diagnosis of sulfur mustard exposure in the human skin the suitability of phage display was explored. Phage display is a relative new method that enables researchers to quickly evaluate a huge range of potentially useful antibodies, thereby bypassing the more costly and time-consuming hybridoma technique. The Tomlinson I and J phage libraries were used to select phage antibodies exhibiting affinity for sulfur mustard adducts on keratins, isolated from human callus. Two kinds of phage antibodies were obtained: antibodies recognizing keratin and antibodies recognizing keratin which was exposed to sulfur mustard. These phage antibodies retained activity after repeated culturing and culturing in larger volumes. For the first time antibody phage display was successfully applied for immunodiagnostics of a chemical warfare agent. © 2007 The Authors.
Subject
Immunodiagnostics
Phage display
Post translational modifications
Chemical warfare agent
Keratin
Keratin antibody
Mustard gas
Antibody affinity
Article
Bacteriophage
Bacterium culture
Callus
Culture technique
Evaluation research
Exposure
Gene library
Human
Human tissue
Hybridoma
Nonhuman
Nucleotide sequence
Phage display
Priority journal
Program development
Protein isolation
Research
Serodiagnosis
Skin
Antibodies, Monoclonal
Bacteriophages
Base Sequence
DNA Primers
Humans
Mustard Gas
Molecular Sequence Numbers: GENBANK: DQ184510, DQ184511, DQ184512, DQ184513;
Chemicals / CAS: mustard gas, 505-60-2; Antibodies, Monoclonal; DNA Primers; Mustard Gas, 505-60-2
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DOI
https://doi.org/10.1111/j.1747-0285.2007.00504.x
TNO identifier
239957
ISSN
1747-0277
Source
Chemical Biology and Drug Design, 69 (5), 314-320
Document type
article