Print Email Facebook Twitter Detection of sulfur mustard adducts in human callus by phage antibodies Title Detection of sulfur mustard adducts in human callus by phage antibodies Author Bikker, F.J. Mars-Groenendijk, R.H. Noort, D. Fidder, A. van der Schans, G.P. TNO Defensie en Veiligheid Publication year 2007 Abstract As part of a research program to develop novel methods for diagnosis of sulfur mustard exposure in the human skin the suitability of phage display was explored. Phage display is a relative new method that enables researchers to quickly evaluate a huge range of potentially useful antibodies, thereby bypassing the more costly and time-consuming hybridoma technique. The Tomlinson I and J phage libraries were used to select phage antibodies exhibiting affinity for sulfur mustard adducts on keratins, isolated from human callus. Two kinds of phage antibodies were obtained: antibodies recognizing keratin and antibodies recognizing keratin which was exposed to sulfur mustard. These phage antibodies retained activity after repeated culturing and culturing in larger volumes. For the first time antibody phage display was successfully applied for immunodiagnostics of a chemical warfare agent. © 2007 The Authors. Subject ImmunodiagnosticsPhage displayPost translational modificationsChemical warfare agentKeratinKeratin antibodyMustard gasAntibody affinityArticleBacteriophageBacterium cultureCallusCulture techniqueEvaluation researchExposureGene libraryHumanHuman tissueHybridomaNonhumanNucleotide sequencePhage displayPriority journalProgram developmentProtein isolationResearchSerodiagnosisSkinAntibodies, MonoclonalBacteriophagesBase SequenceDNA PrimersHumansMustard GasMolecular Sequence Numbers: GENBANK: DQ184510, DQ184511, DQ184512, DQ184513;Chemicals / CAS: mustard gas, 505-60-2; Antibodies, Monoclonal; DNA Primers; Mustard Gas, 505-60-2 To reference this document use: http://resolver.tudelft.nl/uuid:1bdc1206-4d4e-436d-9ac3-8cd733d7762b DOI https://doi.org/10.1111/j.1747-0285.2007.00504.x TNO identifier 239957 ISSN 1747-0277 Source Chemical Biology and Drug Design, 69 (5), 314-320 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.