Print Email Facebook Twitter Low bone mineral density and bone mineral content are associated with low cobalamin status in adolescents Title Low bone mineral density and bone mineral content are associated with low cobalamin status in adolescents Author Dhonukshe-Rutten, R.A.M. van Dusseldorp, M. Schneede, J. de Groot, L.C.P.G.M. van Staveren, W.A. TNO Kwaliteit van Leven Publication year 2005 Abstract Background: Cobalamin deficiency is prevalent in vegetarians and has been associated with increased risk of osteoporosis. Aim of the study: To examine the association between cobalamin status and bone mineral density in adolescents formerly fed a macrobiotic diet and in their counterparts. Methods: In this cross-sectional study bone mineral density (BMD) and bone mineral content (BMC) were determined by DEXA in 73 adolescents (9-15 y) who were fed a macrobiotic diet up to the age of 6 years followed by a lacto-(-ovo-) vegetarian or omnivorous diet. Data from 94 adolescents having consumed an omnivorous diet throughout their lives were used as controls. Serum concentrations of cobalamin, methylmalonic acid (MMA) and homocysteine were measured and calcium intake was assessed by questionnaire. Analysis of covariance (MANCOVA) was performed to calculate adjusted means for vitamin B12 and MMA for low and normal BMC and BMD groups. Results: Serum cobalamin concentrations were significantly lower (geometric mean (GM) 246 pmol/L vs. 469 pmol/L) and MMA concentrations were significantly higher (GM 0.27 μmol/L vs. 0.16 μmol/L) in the formerly macrobiotic-fed adolescents compared to their counterparts. In the total study population, after adjusting for height, weight, bone area, percent lean body mass, age, puberty and calcium intake, serum MMA was significantly higher in subjects with a low BMD (p = 0.0003) than in subjects with a normal BMD. Vitamin B12 was significantly lower in the group with low BMD (p = 0.0035) or BMC (p = 0.0038) than in the group with normal BMD or BMC. When analyses were restricted to the group of formerly macrobiotic-fed adolescents, MMA concentration remained higher in the low BMD group compared to the normal BMD group. Conclusions: In adolescents, signs of an impaired cobalamin status, as judged by elevated concentrations of methylmalonic acid, were associated with low BMD. This was especially true in adolescents fed a macrobiotic diet during the first years of life, where cobalamin deficiency was more prominent. © Steinkopff Verlag 2004. Subject BiologyFood and Chemical Risk AnalysisAdolescentsAnalysis of covarianceBone massCobalamin deficiencyMacrobiotic dietVegetarian dietcalciumcobalamincyanocobalaminhomocysteinemethylmalonic acidadolescenceadolescentage distributionarticlebody heightbody weightbone densitybone mineralcalcium intakecalculationconcentration (parameters)controlled studydietary intakedual energy X ray absorptiometryfeedingfemalefood intakegeometryhealth statushumanhuman experimentlean body weightmacrobiotic dietmalemultivariate analysis of covariancenormal humanpubertyquestionnaireschool childserumvegetarian dietAdolescentAnalysis of VarianceAnthropometryBone DensityCase-Control StudiesChildCross-Sectional StudiesDensitometry, X-RayDiet, MacrobioticFemaleHumansLife StyleMaleMethylmalonic AcidNetherlandsNutritional StatusVitamin B 12Vitamin B 12 DeficiencyVitamin B Complex To reference this document use: http://resolver.tudelft.nl/uuid:1a6e8658-cd11-4d57-b31b-9d0cc7085263 DOI https://doi.org/10.1007/s00394-004-0531-x TNO identifier 238689 ISSN 1436-6207 Source European Journal of Nutrition, 44 (6), 341-347 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.