Title
RPLC-lon-trap-FTMS method for lipid profiling of plasma: Method validation And application to p53 mutant mouse model
Author
Hu, C.
van Dommelen, J.
van der Heijden, R.
Spijksma, G.
Reijmers, T.H.
Wang, M.
Slee, E.
Lu, X.
Xu, G.
van der Greef, J.
Hankemeier, T.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometry method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity (R2, 0.994-1.000), limit of detection (0.08-1.28 μ/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory-The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression. © 2008 American Chemical Society.
Subject
Analytical research
FTMS
Lipid profiling
Lipidomics
Metabolite profiling
Metabolomics
P53 mouse model
Protein p53
Lipid
Article
Controlled study
High performance liquid chromatography
Human
Ion cyclotron resonance mass spectrometry
Ion trap mass spectrometry
Lipid analysis
Lipid blood level
Mouse
Mutational analysis
Nonhuman
Phenotype
Priority journal
Protein expression
Reversed phase liquid chromatography
Animal
Blood
Cyclotron
Infrared spectroscopy
Liquid chromatography
Methodology
Mouse mutant
Reproducibility
Tandem mass spectrometry
Mus
Animals
Chromatography, Liquid
Cyclotrons
Lipids
Mice
Mice, Mutant Strains
Reproducibility of Results
Spectroscopy, Fourier Transform Infrared
Tandem Mass Spectrometry
Tumor Suppressor Protein p53
To reference this document use:
http://resolver.tudelft.nl/uuid:1897bb09-7512-4c36-9d6b-8aee20381113
DOI
https://doi.org/10.1021/pr800373m
TNO identifier
280031
ISSN
1535-3893
Source
Journal of Proteome Research, 7 (11), 4982-4991
Document type
article