Print Email Facebook Twitter RPLC-lon-trap-FTMS method for lipid profiling of plasma: Method validation And application to p53 mutant mouse model Title RPLC-lon-trap-FTMS method for lipid profiling of plasma: Method validation And application to p53 mutant mouse model Author Hu, C. van Dommelen, J. van der Heijden, R. Spijksma, G. Reijmers, T.H. Wang, M. Slee, E. Lu, X. Xu, G. van der Greef, J. Hankemeier, T. TNO Kwaliteit van Leven Publication year 2008 Abstract A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometry method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity (R2, 0.994-1.000), limit of detection (0.08-1.28 μ/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory-The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression. © 2008 American Chemical Society. Subject Analytical researchFTMSLipid profilingLipidomicsMetabolite profilingMetabolomicsP53 mouse modelProtein p53LipidArticleControlled studyHigh performance liquid chromatographyHumanIon cyclotron resonance mass spectrometryIon trap mass spectrometryLipid analysisLipid blood levelMouseMutational analysisNonhumanPhenotypePriority journalProtein expressionReversed phase liquid chromatographyAnimalBloodCyclotronInfrared spectroscopyLiquid chromatographyMethodologyMouse mutantReproducibilityTandem mass spectrometryMusAnimalsChromatography, LiquidCyclotronsLipidsMiceMice, Mutant StrainsReproducibility of ResultsSpectroscopy, Fourier Transform InfraredTandem Mass SpectrometryTumor Suppressor Protein p53 To reference this document use: http://resolver.tudelft.nl/uuid:1897bb09-7512-4c36-9d6b-8aee20381113 DOI https://doi.org/10.1021/pr800373m TNO identifier 280031 ISSN 1535-3893 Source Journal of Proteome Research, 7 (11), 4982-4991 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.