Print Email Facebook Twitter Tolerance controls encephalitogenicity of αB-crystallin in the Lewis rat Title Tolerance controls encephalitogenicity of αB-crystallin in the Lewis rat Author van Stipdonk, M.J.B. Willems, A.A. Plomp, A.C. van Noort, J.M. Boog, C.J.P. TNO Preventie en Gezondheid Publication year 2000 Abstract The myelin-associated protein, αB-crystallin, is considered a candidate autoantigen in multiple sclerosis (MS). In the present study, we examined the potential of αB-crystallin to induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats. Attempts to induce EAE with either bovine, rat or murine αB-crystallin or αB-crystallin peptides consistently failed. Immunization with either autologous rat or murine αB-crystallin did not trigger any antigen-specific T cell response. Immunization with bovine αB-crystallin or a synthetic peptide representing the cryptic epitope 49-64 did trigger T cell responses but these failed to crossreact with autologous rat αB-crystallin. Examination of lymphoid tissues of the Lewis rat revealed constitutive expression of αB-crystallin in thymus, spleen, and peripheral lymphocytes. Our data show that in Lewis rats, constitutive lymphoid expression of αB-crystallin is associated with a state of nonresponsiveness to autologous αB-crystallin that effectively controls the development of EAE in response to this myelin antigen. Copyright (C) 2000 Elsevier Science B.V. Chemicals/CAS: Autoantigens; Crystallins; Immunodominant Epitopes; Peptide Fragments; RNA, Messenger Subject HealthToleranceAutoantigenCrystallinMyelinMyelin associated glycoproteinAllergic encephalomyelitisAnimal cellAnimal experimentAutoimmune diseaseCentral nervous systemControlled studyImmunizationLymphocyte proliferationMultiple sclerosisNonhumanRat strainT lymphocyteAnimalsAutoantigensCattleCrystallinsDose-Response Relationship, ImmunologicEncephalomyelitis, Autoimmune, ExperimentalHypersensitivity, DelayedImmune ToleranceImmunity, CellularImmunodominant EpitopesLymphoid TissueMaleMiceOrgan SpecificityPeptide FragmentsPhosphorylationRatsRats, Inbred LewReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSpecies SpecificityT-Lymphocytes To reference this document use: http://resolver.tudelft.nl/uuid:142cdd12-cdcb-4086-8a76-ce3cdcd163ea DOI https://doi.org/10.1016/s0165-5728(99)00171-x TNO identifier 235422 ISSN 0165-5728 Source Journal of Neuroimmunology, 103 (2), 103-111 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.