Title
Tolerance controls encephalitogenicity of αB-crystallin in the Lewis rat
Author
van Stipdonk, M.J.B.
Willems, A.A.
Plomp, A.C.
van Noort, J.M.
Boog, C.J.P.
TNO Preventie en Gezondheid
Publication year
2000
Abstract
The myelin-associated protein, αB-crystallin, is considered a candidate autoantigen in multiple sclerosis (MS). In the present study, we examined the potential of αB-crystallin to induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats. Attempts to induce EAE with either bovine, rat or murine αB-crystallin or αB-crystallin peptides consistently failed. Immunization with either autologous rat or murine αB-crystallin did not trigger any antigen-specific T cell response. Immunization with bovine αB-crystallin or a synthetic peptide representing the cryptic epitope 49-64 did trigger T cell responses but these failed to crossreact with autologous rat αB-crystallin. Examination of lymphoid tissues of the Lewis rat revealed constitutive expression of αB-crystallin in thymus, spleen, and peripheral lymphocytes. Our data show that in Lewis rats, constitutive lymphoid expression of αB-crystallin is associated with a state of nonresponsiveness to autologous αB-crystallin that effectively controls the development of EAE in response to this myelin antigen. Copyright (C) 2000 Elsevier Science B.V. Chemicals/CAS: Autoantigens; Crystallins; Immunodominant Epitopes; Peptide Fragments; RNA, Messenger
Subject
Health
Tolerance
Autoantigen
Crystallin
Myelin
Myelin associated glycoprotein
Allergic encephalomyelitis
Animal cell
Animal experiment
Autoimmune disease
Central nervous system
Controlled study
Immunization
Lymphocyte proliferation
Multiple sclerosis
Nonhuman
Rat strain
T lymphocyte
Animals
Autoantigens
Cattle
Crystallins
Dose-Response Relationship, Immunologic
Encephalomyelitis, Autoimmune, Experimental
Hypersensitivity, Delayed
Immune Tolerance
Immunity, Cellular
Immunodominant Epitopes
Lymphoid Tissue
Male
Mice
Organ Specificity
Peptide Fragments
Phosphorylation
Rats
Rats, Inbred Lew
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Species Specificity
T-Lymphocytes
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DOI
https://doi.org/10.1016/s0165-5728(99)00171-x
TNO identifier
235422
ISSN
0165-5728
Source
Journal of Neuroimmunology, 103 (2), 103-111
Document type
article