Title
Comparison of five in vitro digestion models to in vivo experimental results: Lead bioaccessibility in the human gastrointestinal tract
Author
van de Wiele, T.R.
Oomen, A.G.
Wragg, J.
Cave, M.
Minekus, M.
Hack, A.
Cornelis, C.
Rompelberg, C.J.M.
de Zwart, L.L.
Klinck, B.
van Wijnen, J.
Verstraete, W.
Sips, A.J.A.M.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
This paper presents a multi-laboratory comparison study of in vitro models assessing bioaccessibility of soil-bound lead in the human gastrointestinal tract under simulated fasted and fed conditions. Oral bioavailability data from a previous human in vivo study on the same soil served as a reference point. In general, the bioaccessible lead fraction was significantly (P < 0.05) different between the in vitro methods and ranged for the fasted models from 2% to 33% and for the fed models from 7% to 29%. The in vivo bioavailability data from literature were 26.2 ± 8.1% for fasted conditions, compared to 2.5 ± 1.7% for fed conditions. Under fed conditions, all models returned higher bioaccessibility values than the in vivo bioavailability; whereas three models returned a lower bioaccessibility than bioavailability under fasted conditions. These differences are often due to the method's digestion parameters that need further optimization. An important outcome of this study was the determination that the method for separating the bioaccessible lead from the non-bioaccessible fraction (centrifugation, filtration, ultrafiltration) is crucial for the interpretation of the results. Bioaccessibility values from models that use more stringent separation methods better approximate in vivo bioavailability results, yet at the expense of the level of conservancy. We conclude from this study that more optimization of in vitro digestion models is needed for use in risk assessment. Moreover, attention should be paid to the laboratory separation method since it largely influences what fraction of the contaminant is considered bioaccessible. Copyright © Taylor & Francis Group, LLC.
Subject
Biomedical Research
Bioaccessibility
Bioavailable
Human gut
In vitro digestion
Intestine
Pb
Soil ingestion
Approximation algorithms
Biological organs
Computer simulation
Lead
Optimization
Risk assessment
Separation
Soils
Bioaccessibility
Bioavailability
Gastrointestinal tract
Human gut
In vitro digestion
Soil ingestion
Physiological models
lead
article
bioavailability
centrifugation
comparative study
diet restriction
digestion
filtration
food intake
gastrointestinal tract
in vitro study
lead poisoning
simulation
soil analysis
ultrafiltration
Biological Availability
Data Interpretation, Statistical
Gastrointestinal Tract
Humans
Lead
Models, Biological
Soil
Soil Pollutants
To reference this document use:
http://resolver.tudelft.nl/uuid:12c2cdf3-afff-4096-8926-7553e76ffbfa
DOI
https://doi.org/10.1080/10934520701434919
TNO identifier
239786
ISSN
1093-4529
Source
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, 42 (9), 1203-1211
Document type
article