Print Email Facebook Twitter Determination of the relationship between group A streptococcal genome content, M type, and toxic shock syndrome by a mixed genome microarray Title Determination of the relationship between group A streptococcal genome content, M type, and toxic shock syndrome by a mixed genome microarray Author Vlaminckx, B.J.M. Schuren, F.H.J. Montijn, R.C. Caspers, M.P.M. Fluit, A.C. Wannet, W.J.B. Schouls, L.M. Verhoef, J. Jansen, W.T.M. TNO Kwaliteit van Leven Publication year 2007 Abstract Group A streptococci (GAS), or Streptococcus pyogenes, are associated with a remarkable variety of diseases, ranging from superficial infections to life-threatening diseases such as toxic-shock-like syndrome (TSS). GAS strains belonging to M types M1 and M3 are associated with TSS. This study aims to obtain insight into the gene profiles underlying different M types and disease manifestations. Genomic differences between 76 clinically well characterized GAS strains collected in The Netherlands were examined using a mixed-genome microarray. Inter-M-type genomic differences clearly outweighed intra-M-type genome variation. Phages were major contributors to observed genome diversification. We identified four novel genes, including two genes encoding fibronectin-binding-like proteins, which are highly specific to a subset of M types and thus may contribute to M-type-associated disease manifestations. All M12 strains were characterized by the unique absence of the citrate lyase complex and reduced growth under hypoxic, nutrient-deprived conditions. Furthermore, six virulence factors, including genes encoding a complement-inhibiting protein (sic), an exotoxin (speA), iron(III) binding factor, collagen binding factor (cpa), and fibrinogen binding factor (prt2-like), were unique to M1 and/or M3 strains. These virulence factors may contribute to the potential of these strains to cause TSS. Finally, in contrast to M-type-specific virulence profiles, we did not identify a common virulence profile among strains associated with TSS irrespective of their M type. Copyright © 2007, American Society for Microbiology. All Rights Reserved. Subject BiologyBiotechnologycitric acidcollagen binding factorcomplement inhibiting proteinexotoxinferric ionfibrinogen binding factoriron binding proteinlyasespeA proteinvirulence factorarticlebacterial strainbacterial virulencebacteriophagecontrolled studyDNA fingerprintingDNA microarraygene identificationgenetic associationgenetic codegenetic variabilitygenomegenomicshumanhypoxiamajor clinical studymicrobial diversityNetherlandsnucleotide sequencepriority journalStreptococcus group Atoxic shock syndromeAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsCarrier ProteinsElectrophoresis, Gel, Pulsed-FieldGene Expression ProfilingGenome, BacterialHumansImage Processing, Computer-AssistedOligonucleotide Array Sequence AnalysisShock, SepticStreptococcal InfectionsStreptococcus pyogenesVirulence Factors To reference this document use: http://resolver.tudelft.nl/uuid:0f786367-f464-4e8e-8cf3-6335f0d35571 DOI https://doi.org/10.1128/iai.01291-06 TNO identifier 239950 ISSN 0019-9567 Source Infection and Immunity, 75 (5), 2603-2611 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.