Title
Determination of the relationship between group A streptococcal genome content, M type, and toxic shock syndrome by a mixed genome microarray
Author
Vlaminckx, B.J.M.
Schuren, F.H.J.
Montijn, R.C.
Caspers, M.P.M.
Fluit, A.C.
Wannet, W.J.B.
Schouls, L.M.
Verhoef, J.
Jansen, W.T.M.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
Group A streptococci (GAS), or Streptococcus pyogenes, are associated with a remarkable variety of diseases, ranging from superficial infections to life-threatening diseases such as toxic-shock-like syndrome (TSS). GAS strains belonging to M types M1 and M3 are associated with TSS. This study aims to obtain insight into the gene profiles underlying different M types and disease manifestations. Genomic differences between 76 clinically well characterized GAS strains collected in The Netherlands were examined using a mixed-genome microarray. Inter-M-type genomic differences clearly outweighed intra-M-type genome variation. Phages were major contributors to observed genome diversification. We identified four novel genes, including two genes encoding fibronectin-binding-like proteins, which are highly specific to a subset of M types and thus may contribute to M-type-associated disease manifestations. All M12 strains were characterized by the unique absence of the citrate lyase complex and reduced growth under hypoxic, nutrient-deprived conditions. Furthermore, six virulence factors, including genes encoding a complement-inhibiting protein (sic), an exotoxin (speA), iron(III) binding factor, collagen binding factor (cpa), and fibrinogen binding factor (prt2-like), were unique to M1 and/or M3 strains. These virulence factors may contribute to the potential of these strains to cause TSS. Finally, in contrast to M-type-specific virulence profiles, we did not identify a common virulence profile among strains associated with TSS irrespective of their M type. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Subject
Biology
Biotechnology
citric acid
collagen binding factor
complement inhibiting protein
exotoxin
ferric ion
fibrinogen binding factor
iron binding protein
lyase
speA protein
virulence factor
article
bacterial strain
bacterial virulence
bacteriophage
controlled study
DNA fingerprinting
DNA microarray
gene identification
genetic association
genetic code
genetic variability
genome
genomics
human
hypoxia
major clinical study
microbial diversity
Netherlands
nucleotide sequence
priority journal
Streptococcus group A
toxic shock syndrome
Antigens, Bacterial
Bacterial Outer Membrane Proteins
Bacterial Proteins
Carrier Proteins
Electrophoresis, Gel, Pulsed-Field
Gene Expression Profiling
Genome, Bacterial
Humans
Image Processing, Computer-Assisted
Oligonucleotide Array Sequence Analysis
Shock, Septic
Streptococcal Infections
Streptococcus pyogenes
Virulence Factors
To reference this document use:
http://resolver.tudelft.nl/uuid:0f786367-f464-4e8e-8cf3-6335f0d35571
DOI
https://doi.org/10.1128/iai.01291-06
TNO identifier
239950
ISSN
0019-9567
Source
Infection and Immunity, 75 (5), 2603-2611
Document type
article