Print Email Facebook Twitter The metabolic activity of fecal microbiota from healthy individuals and patients with inflammatory bowel disease Title The metabolic activity of fecal microbiota from healthy individuals and patients with inflammatory bowel disease Author van Nuenen, M.H.M.C. Venema, K. van der Woude, J.C.J. Kuipers, E.J. TNO Voeding Publication year 2004 Abstract The hypothesis was studied that intestinal microbial metabolites play a role in the pathogenesis of inflammatory bowel disease. For that purpose, an in vitro model of the colon was inoculated with fresh feces of six healthy individuals and eight inflammatory bowel disease patients. Samples were taken from the model over time to analyze metabolites from both saccharolytic and proteolytic fermentation. Microbiotas from inflammatory bowel disease patients produced significantly more short-chain fatty acids and ammonia than microbiotas from healthy individuals. Furthermore, the branched-chain fatty acid production was 25% higher after inoculation with microbiotas from patients than after inoculation with microbiotas from healthy individuals. Phenolic compounds were produced by all microbiotas, with large interindividual variation. The production of (potentially toxic) metabolites may play a role in the onset or chronicity of inflammatory bowel disease, because they were produced in higher amounts by microbiotas from these patients than by microbiotas from healthy individuals. Subject NutritionPhysiological SciencesFermentationIn vitro modelInflammatory bowel diseaseMetabolitesMicrobial activityammoniabranched chain fatty acidfatty acidlactic acidphenol derivativeshort chain fatty acidunclassified drugadultarticlecarbohydrate metabolismchronicityclinical articlecontrolled studyenteritisfatty acid synthesisfeces microflorafermentationhumanin vitro studymetabolitemicrobial metabolismmodelnonhumannormal humanpriority journalprotein degradationAdultAmmoniaBacteriaColitis, UlcerativeColonCrohn DiseaseFatty AcidsFatty Acids, VolatileFecesFermentationHumansLactic AcidMiddle Aged To reference this document use: http://resolver.tudelft.nl/uuid:0954d11f-37fd-40c1-a7c8-9063f2106bba DOI https://doi.org/10.1023/b:ddas.0000020508.64440.73 TNO identifier 237660 ISSN 0163-2116 Source Digestive Diseases and Sciences, 49 (3), 485-491 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.