Title
Lactobacillus casei Shirota does not decrease the food allergic response to peanut extract in Brown Norway rats
Author
de Jonge, J.D.
Ezendam, J.
Knippels, L.M.J.
Penninks, A.H.
Pieters, R.
van Loveren, H.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
Probiotics are claimed to beneficially affect the immune system and their involvement in allergy prevention is being investigated extensively. However, the efficacy of probiotics in allergy prevention remains controversial. We investigated whether the probiotic Lactobacillus casei Shirota (LcS) could modulate the food allergic response against peanut extract (PE) in Brown Norway (BN) rats. For this purpose BN rats were sensitized to PE (0, 1 and 10 mg/(rat d)) by daily oral gavage and the LcS-groups were additionally orally dosed with 1 × 109 colony forming units LcS/(rat d). LcS administration had minor effects in animals that were not sensitized. LcS increased Th1-(PE-specific IgG1), whereas the Th1/Th2 ratio based on PE-specific IgG1/PE-specific IgG2a shifted towards Th2 dominance in rats sensitized to PE in the presence of LcS as compared to rats that were sensitized to PE only. LcS stimulated PE-specific IgG2a; but for PE-specific IgE the effect was less clear; whereas there was no overall effect, two rats did not show detectable specific IgE antibodies, whereas the remainder showed significantly increased levels. LcS also resulted in increased numbers of basophilic granulocytes in blood. Furthermore, LcS increased levels of both Th1-(IFN-γ) and Th2-(IL-4) related cytokines in PE stimulated spleen and mesenteric lymph node (MLN) cells, but predominantly IL-4 levels in the supernatants of both spleens and MLNs. Our study does not support the hypothesis that LcS down-regulates food allergic responses in a BN rat model for food allergy to peanut. © 2008 Elsevier Ireland Ltd. All rights reserved.
Subject
Nutrition
Brown Norway rat
Food allergy
Lactobacillus casei Shirota
Peanut
arachis oil
gamma interferon
immunoglobulin E antibody
immunoglobulin G2a
interleukin 4
mast cell protease 2
probiotic agent
serine proteinase
animal cell
animal experiment
animal model
antibody blood level
antibody titer
article
bacterial strain
basophil
brown Norway rat
controlled study
cytokine production
drug effect
female
Lactobacillus casei
mesentery lymph node
nonhuman
peanut allergy
priority journal
provocation test
rat
sensitization
spleen cell
Th1 cell
Th2 cell
Animals
Basophils
Cell Count
Chymases
Cytokines
Digoxigenin
Endotoxins
Immunoglobulin E
Immunoglobulin G
Lactobacillus casei
Leukocyte Count
Peanut Hypersensitivity
Plant Extracts
Probiotics
Rats
Rats, Inbred BN
Th1 Cells
Th2 Cells
Animalia
Arachis hypogaea
Lactobacillus casei
Rattus
Rattus norvegicus
To reference this document use:
http://resolver.tudelft.nl/uuid:083fc566-9271-4b72-9dce-3195c00d7fa5
DOI
https://doi.org/10.1016/j.tox.2008.04.016
TNO identifier
240921
ISSN
0300-483X
Source
Toxicology, 249 (2-3), 140-145
Document type
article