Print Email Facebook Twitter The effect of the undigested fraction of maize products on the activity and composition of the microbiota determined in a dynamic in vitro model of the human proximal large intestine. Title The effect of the undigested fraction of maize products on the activity and composition of the microbiota determined in a dynamic in vitro model of the human proximal large intestine. Author Maathuis, A. Hoffman, A. Evans, A. Sanders, L. Venema, K. TNO Kwaliteit van Leven Publication year 2009 Abstract OBJECTIVE: To investigate the effect of 5 newly developed maize-based fibers on the activity and composition of the microbiota in the colon. The fibers tested were glucose-based and had variable structures, including 2 resistant starch preparations, soluble corn fiber, pullulan, and soluble fiber dextrin. METHODS: The fibers were predigested, mono- and disaccharides were removed, and the residual polymer was used to assess the production of microbial metabolites and changes in composition of the microbiota using a dynamic, validated, in vitro model of the large intestine. RESULTS: Microbial metabolite analysis showed an increase in short-chain fatty acids for all fibers, with varying levels of butyrate production for each fiber. The greatest increase of butyrate, both in terms of absolute amounts and as a proportion of total short-chain fatty acids, was observed for pullulan. All fibers also reduced toxic metabolites from protein fermentation compared to the poorly fermentable control (cellulose). Microbial composition was assessed using a micro-array platform. All fibers showed increases of bifidobacteria and some Lactobacillus species, although different species were stimulated by different fibers. Pullulan showed the largest increase of bifidobacteria. CONCLUSIONS: All fibers showed prebiotic activity in terms of increases in growth and/or activity of beneficial microbes. In addition, compared to the control, health-promoting metabolites were produced in higher amounts, while putrefactive metabolites were reduced for all fibers. The importance of the findings lies in the fact that the newly developed, maize-based fibers shift the intestinal environment to a healthier milieu, with increased health-promoting metabolites and health-beneficial microbes. Subject BiologyBiomedical ResearcharticleBifidobacteriumdigestionhumanlarge intestinemaizemetabolismmetagenomemicrobiologyphysiologyBifidobacteriumDigestionHumansIntestine, LargeMetagenomeZea mays To reference this document use: http://resolver.tudelft.nl/uuid:0621acd2-63e6-46cd-85fb-809d980d321b TNO identifier 409311 ISSN 1541-1087 Source Journal of the American College of Nutrition, 28 (6), 657-666 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.