Title
Metabolomics (liver and blood profiling) in a mouse model in response to fasting: A study of hepatic steatosis
Author
van Ginneken, V.
Verhey, E.
Poelmann, R.
Ramakers, R.
van Dijk, K.W.
Ham, L.
Voshol, P.
Havekes, L.
van Eck, M.
van der Greef, J.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
A metabolomic approach was applied to a mouse model of starvation-induced hepatic steatosis. After 24 h of fasting it appears that starvation reduced the phospholipids (PL), free cholesterol (FC), and cholesterol esters (CE) content of low-density lipoproteins (LDL). In liver lipid profiles major changes were observed using different techniques. High performance thin layer chromatography (HPTLC)-measurements of liver-homogenates indicated a significant rise of FC with 192%, triacylglycerols (TG) with 456% and cholesterol esters (CE) with 268% after 24 h of starvation in comparison with the control group. Reversed phase liquid chromatography coupled to mass spectrometry measurements (LC-MS) of liver homogenate indicated that the intensity of Phosphatidylcholine (PC) in the 24-h starvation group dropped to 90% of the value in the control group while the intensity of CE and TG increased to 157% and 331%, respectively, of the control group. Interestingly, a 49:4-TG with an odd number of C atoms appeared during starvation. This unique triacylglycerol has all characteristics of a biomarker for detection of hepatic steatosis. These observations indicate that in mammals liver lipid profiles are a dynamic system which are readily modulated by environmental factors like starvation. © 2007 Elsevier B.V. All rights reserved.
Subject
Analytical research
Biomedical research
Hepatic steatosis
Lipids
Liver
Metabolomics
Starvation
Triacylglycerols
cholesterol
cholesterol ester
low density lipoprotein cholesterol
phosphatidylcholine
phospholipid derivative
triacylglycerol
animal experiment
animal model
animal tissue
article
blood examination
controlled study
diet restriction
fatty liver
high performance thin layer chromatography
lipid blood level
lipid storage
liver homogenate
male
mass spectrometry
metabolomics
mouse
nonhuman
priority journal
reversed phase liquid chromatography
starvation
Animals
Blood
Cholesterol Esters
Chromatography, High Pressure Liquid
Disease Models, Animal
Fatty Liver
Food Deprivation
Lipoproteins, LDL
Liver
Male
Mice
Mice, Inbred C57BL
Phosphatidylcholines
Phospholipids
Triglycerides
Mammalia
To reference this document use:
http://resolver.tudelft.nl/uuid:04a14123-b5ef-4a3a-8cd3-9da8dfd65434
DOI
https://doi.org/10.1016/j.bbalip.2007.07.007
TNO identifier
240207
ISSN
1388-1981
Source
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1771 (10), 1263-1270
Document type
article