Title
Assessing the zearalenone-binding activity of adsorbent materials during passage through a dynamic in vitro gastrointestinal model
Author
Avantaggiato, G.
Havenaar, R.
Visconti, A.
TNO Voeding
Publication year
2003
Abstract
A novel approach is presented herein to study the intestinal absorption of mycotoxins by using a laboratory model that mimics the metabolic processes of the gastrointestinal (GI) tract of healthy pigs. This model was used to evaluate the small-intestinal absorption of zearalenone from contaminated wheat (4.1 mg/kg) and the effectiveness of activated carbon and cholestyramine at four inclusion levels (0.25, 0.5, 1 and 2%) in reducing toxin absorption. Approximately 32% of ZEA intake (247 μg) was released from the food matrix during 6 h of digestion and was rapidly absorbed at intestinal level. A significant reduction of intestinal absorption of ZEA was found after inclusion of activated carbon or cholestyramine, even at the lowest dose of adsorbents, with a more pronounced effect exhibited by activated carbon. In particular, when 2% of activated carbon or cholestyramine was added to the meal the ZEA intestinal absorption was lowered from 32% of ZEA intake to 5 and 16%, respectively. The sequestering effect of both adsorbents took place already during the first 2 h of digestion and persisted during the rest of the experiment. The GI-model is a rapid and physiologically relevant method to test the efficacy of adsorbent materials in binding mycotoxins and can be used to pre-screen mycotoxin/adsorbent combinations as an alternative to animal experiments. © 2003 Elsevier Ltd. All rights reserved.
Subject
Health
Physiological Sciences
Activated carbon
Cholestyramine
Gastrointestinal model
Mycotoxins
Zearalenone
activated carbon
adsorbent
colestyramine
zearalenone
anion exchange resin
charcoal
article
binding affinity
digestion
food contamination
model
small intestine absorption
swine
wheat
absorption
animal
bile
biological model
chemistry
digestive system
intestine absorption
metabolism
pancreas
physiology
Animalia
Triticum aestivum
Absorption
Animals
Anion Exchange Resins
Bile
Charcoal
Cholestyramine
Digestive System
Food Contamination
Intestinal Absorption
Models, Biological
Pancreas
Swine
Triticum
Zearalenone
To reference this document use:
http://resolver.tudelft.nl/uuid:042a1f14-62cc-493c-9685-910ae3b8c96b
DOI
https://doi.org/10.1016/s0278-6915(03)00113-3
TNO identifier
237299
ISSN
0278-6915
Source
Food and Chemical Toxicology, 41 (10), 1283-1290
Document type
article