Title
Immunologic activity of schistosomal and bacterial TLR2 ligands in Gabonese children
Author
Retra, K.
van Riet, E.
Adegnika, A.A.
Everts, B.
van Geest, S.
Kremsner, P.G.
van Hellemond, J.J.
van der Kleij, D.
Tielens, A.G.M.
Yazdanbakhsh, M.
TNO Defensie en Veiligheid
Publication year
2008
Abstract
Schistosomes carry lipid moieties that interact with the immune system. To understand the consequence of interactions in terms of polarizing the cytokine profiles, the effect of two Toll-like receptor-2 (TLR2) activating schistosomal lipid fractions was studied on whole blood from Gabonese children living in a schistosomiasis endemic area. One fraction contained lysophosphatidylserine [monoacylglycerophosphoserine (lysoGPSer)] plus diacylphosphatidylserine [diacylglycerophosphoserine (GPSer)] while the other contained lysoGPSer and only a trace of GPSer. The effect of these schistosomal lipid fractions was compared with the known bacterial TLR2 ligands PAM3CSK4 and MALP-2. PAM3CSK4 and MALP-2 had preferential IL-10-activating capacities, while the fraction containing lysoGPSer plus GPSer had a strong TNF-α-inducing capacity. The fraction containing lysoGPSer was neutral with respect to pro- vs. anti-inflammatory effects. When Th1 and Th2 cytokines were analysed, the schistosomal lipid fraction containing lysoGPSer plus GPSer showed a stronger Th2 response compared to PAM3CSK 4, MALP-2 and lysoGPSer alone. Therefore, the study indicates that not only TLR2 ligands derived from bacteria or from parasites can generate distinct cytokine profiles but also that the composition of lipid entities reaching the immune system can be important in leading to different immune outcomes. This information may be important for exploitation of immune modulatory molecules. © 2008 The Authors.
Subject
Life
CBRN - CBRN Protection
ELSS - Earth, Life and Social Sciences
Biology
Lysophosphatidylserine
MALP-2
Monoacylglycerophosphoserine
PAM 3CSK4
Schistosoma mansoni
Th1
Th2
Toll-like receptor
Interleukin 10
Ligand
Lysophosphatidylserine
Phosphatidylserine
Toll like receptor 2
Tumor necrosis factor alpha
Adolescent
Blood culture
Child
Clinical article
Endemic disease
Female
Humans
Immune system
Immunoreactivity
Immunoregulation
Lipid analysis
Male
Schistosoma
Schistosomiasis
Statistical analysis
Animals
Cell Line
Child, Preschool
Cytokines
Gabon
Lysophospholipids
Oligopeptides
Peptides
Schistosoma mansoni
Toll-Like Receptor 2
Bacteria (microorganisms)
Schistosoma mansoni
Toll like receptor 2, 203811-81-8
Cytokines
Lysophosphatidylserine
Macrophage stimulatory lipopeptide 2
Pam(3)CSK(4) peptide
To reference this document use:
http://resolver.tudelft.nl/uuid:0421be9c-c783-4c6b-b6a2-1958b6eb06f2
DOI
https://doi.org/10.1111/j.1365-3024.2007.01000.x
TNO identifier
240572
ISSN
0141-9838
Source
Parasite Immunology, 30 (1), 39-46
Document type
article