Title
PET imaging of disease progression and treatment effects in the experimental autoimmune encephalomyelitis rat model
Author
de Paula Faria, D.
Vlaming, M.L.H.
Copray, S.C.V.M.
Tielen, F.
Anthonijsz, H.J.A.
Sijbesma, J.W.A.
Buchpiguel, C.A.
Dierckx, R.A.J.O.
van der Hoorn, J.W.A.
de Vries, E.F.J.
Publication year
2014
Abstract
The experimental autoimmune encephalomyelitis model is a model of multiple sclerosis that closely mimics the disease characteristics in humans. The main hallmarks of multiple sclerosis are neuroinflammation (microglia activation, monocyte invasion, and T-cell infiltration) and demyelination. PET imaging may be a useful noninvasive technique for monitoring disease progression and drug treatment efficacy in vivo. Methods: Experimental autoimmune encephalomyelitis was induced by myelin-oligodendrocyte glycoprotein immunization in female Dark Agouti rats. Experimental autoimmune encephalomyelitis rats were imaged at baseline and at days 6, 11, 15, and 19 after immunization to monitor monocyte and microglia activation (11C-PK11195) and demyelination ( 11C-MeDAS) during normal disease progression and during treatment with dexamethasone. Results: 11C-PK11195 PET detected activation of microglia and monocytes in the brain stem and spinal cord during disease progression. The uptake of 11C-PK11195 was elevated in dexamethasone-treated animals that had shown mild clinical symptoms that had resolved at the time of imaging. Demyelination was not detected by 11C-MeDAS PET, probably because of the small size of the lesions (average, 0.13 mm). Conclusion: PET imaging of neuroinflammation can be used to monitor disease progression and the consequences of treatment in the experimental autoimmune encephalomyelitis rat model. PET imaging was more sensitive than clinical symptoms for detecting inflammatory changes in the central nervous system. COPYRIGHT © 2014 by the Society of Nuclear Medicine and Molecular Imaging, Inc.Chemicals/CAS: dexamethasone acetate, 1177-87-3
Subject
Life
MSB - Microbiology and Systems Biology MHR - Metabolic Health Research
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Demyelination
Multiple sclerosis
Neuroinflammation
PET imaging
Dexamethasone acetate
MeDAS c 11
Myelin oligodendrocyte glycoprotein
N sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide c 11
Radiopharmaceutical agent
Unclassified drug
Animal cell
Animal experiment
Animal model
Animal tissue
Brain region
Cell invasion
Controlled study
Demyelination
Disease course
Drug effect
Experimental autoimmune encephalomyelitis
Female
In vivo study
Microglia
Monocyte
Neuroimaging
Nonhuman
PET scanner
Positron emission tomography
Priority journal
Rat
Scoring system
To reference this document use:
http://resolver.tudelft.nl/uuid:01f6aa40-bc61-4696-9d49-58d01c02f699
DOI
https://doi.org/10.2967/jnumed.114.137216
TNO identifier
513449
ISSN
0161-5505
Source
Journal of Nuclear Medicine, 55 (8), 1330-1335
Document type
article