Title
Steady progression of osteoarthritic features in the canine groove model
Author
Marijnissen, A.C.A.
van Roermund, P.M.
Verzijl, N.
Tekoppele, J.M.
Bijlsma, J.W.J.
Lafeber, F.P.J.G.
TNO Preventie en Gezondheid
Publication year
2002
Abstract
Objective: Recently we described a canine model of osteoarthritis (OA), the groove model with features of OA at 10 weeks after induction, identical to those seen in the canine anterior cruciate ligament transection (ACLT) model. This new model depends on cartilage damage accompanied by transient intensified loading of the affected joint. The present study evaluates this groove model at 20 and 40 weeks after induction, to assess whether the osteoarthritic features progress in time. Methods: Grooves were made in the femoral condyles of one knee without damaging the subchondral bone. After surgery the dogs were forced to load the experimental joint 3 days per week (4 hours/day) for 20 weeks by fixing the contralateral control limb to the trunk. After 20 weeks and 40 weeks (the last 20 weeks normal loading) joints were analysed for biochemical and histological features of OA. Results: All biochemical cartilage parameters were indicative of OA and all these parameters suggested a slow progression of degeneration over time from 20 to 40 weeks after induction, statistically significant for synthesis and content of proteoglycans as well as Mankin grade. Synovial inflammation, which was mild, diminished slightly in time. Conclusion: The degenerative joint damage in the canine groove model is slowly progressive over time in the first year. The cartilage degeneration is induced by a one-time trauma and is not primarily mediated by synovial inflammation, which gives this model unique characteristics compared to presently available models for studying early osteoarthritic features in vivo. In the groove model the effect of treatment of cartilage damage is not counteracted by permanent joint instability or hampered by inflammation. Therefore, the model might be more sensitive to detect effects of therapy, aimed at cartilage protection and repair. © 2002 Published by Elsevier Science Ltd on behalf of OsteoArthritis Research Society International.Chemicals/CAS: Proteoglycans
Subject
Biology
Biomedical Research
Canine model
Cartilage
Osteoarthritis
Proteoglycans
proteoglycan
Animal experiment
Animal model
Bone injury
Cartilage degeneration
Controlled study
Disease course
Femur condyle
Histopathology
In vivo study
Joint degeneration
Joint injury
Joint stability
Knee osteoarthritis
Laboratory test
Loading test
Nonhuman
Proteoglycan synthesis
Surgical technique
Synovitis
Treatment outcome
Animals
Cartilage, Articular
Disease Models, Animal
Dogs
Female
Joints
Osteoarthritis
Proteoglycans
Synovial Membrane
Time Factors
To reference this document use:
http://resolver.tudelft.nl/uuid:011b9344-d66c-4c77-a023-fdc5b6e313ae
DOI
https://doi.org/10.1053/joca.2001.0507
TNO identifier
236549
ISSN
1063-4584
Source
Osteoarthritis and Cartilage, 10 (4), 282-289
Document type
article