Title
Biological production of monoethanolamine by engineered Pseudomonas putida S12
Author
Foti, M.J.
Médici, R.
Ruijssenaars, H.J.
Publication year
2013
Abstract
Pseudomonas putida S12 was engineered for the production of monoethanolamine (MEA) from glucose via the decarboxylation of the central metabolite l-serine, which is catalyzed by the enzyme l-serine decarboxylase (SDC).The host was first evaluated for its tolerance towards MEA as well as its endogenous ability to degrade this alkanolamine. Growth inhibition was observed at MEA concentrations above 100. mM, but growth was never completely arrested even at 750. mM of MEA. P. putida S12 was able to catabolize MEA in the absence of ammonia, but deletion of the eutBC genes that encode ethanolamine ammonia-lyase (EAL) enzyme sufficed to eliminate this capacity.For the biological production of MEA, the sdc genes from Arabidopsis thaliana (full-length and a truncated version) and Volvox carteri were expressed in P. putida S12. From 20mM of glucose, negligible amounts of MEA were produced by P. putida S12 δeutBC expressing the sdc genes from A. thaliana and V. carteri. However, 0.07mmol of MEA was obtained per g of cell dry weight of P. putida S12 δeutBC expressing the truncated variant of the A. thaliana SDC. When the medium was supplemented with l-serine (30mM), MEA production increased to 1.25mmolMEAg-1 CDW, demonstrating that l-serine availability was limiting MEA production. © 2013 Published by Elsevier B.V.
Subject
Life
FI - Functional Ingredients
EELS - Earth, Environmental and Life Sciences
Biomedical Innovation
Biology
Healthy Living
Amino acid decarboxylation
Ethanolamine ammonia lyase (EAL)
L-Serine decarboxylase (SDC)
Monoethanolamine (MEA)
Pseudomonas putida S12
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http://resolver.tudelft.nl/uuid:fecbf3c2-b84b-4cda-b332-5e972eb37416
TNO identifier
478188
Publisher
Elsevier, Amsterdam
ISSN
0168-1656
Source
Journal of Biotechnology, 167 (3), 344-349
Document type
article