Title
Evidence for linkage of 3-phosphoglycerate kinase, hypoxanthine-guanine-phosphoribosyl transferase, and glucose 6-phosphate dehydrogenase loci in Chinese hamster cells studied by using a relationship between gene multiplicity and enzyme activity
Author
Medisch Biologisch Labortorium TNO
Westerveld, A.
Visser, R.P.L.S.
Freeke, M.A.
Bootsma, D.
Publication year
1972
Abstract
The PGK activity was assayed in diploid, hyperdiploid, tetraploid, and hybrid cells all originating from the same Chinese hamster cell line (DON line). A relationship between gene multiplicity and enzyme activity was observed. Selective pressure on the HGPRT locus by growth of hybrid cells in the presence of 8-azaguanine resulted in decreased levels of PGK activity. Growth of these hybrids in the presence of 5-BUdR did not influence the enzyme activity. It was concluded that the genes coding for HGPRT, PGK, and G6PD are linked in the Chinese hamster. The TK locus seems to be linked neither to the HGPRT, PGK, and G6PD loci nor to the 6PGD locus. © 1972 Plenum Publishing Corporation.
Subject
8 azaguanine
Broxuridine
Glucose 6 phosphate dehydrogenase
Glycosyltransferase
Guanine
Hypoxanthine derivative
Phosphoglycerate kinase
Thymidine kinase
Animal
Cell count
Cell line
Drug effect
Drug resistance
Enzymology
Gene
Genetic linkage
Hamster
Hybrid cell
Lung
Polyploidy
Spectrophotometry
Technique
Azaguanine
Bromodeoxyuridine
Genes
Glucosephosphate Dehydrogenase
Guanine
Hamsters
Hybrid Cells
Hypoxanthines
Linkage (Genetics)
Lung
Methods
Pentosyltransferases
Phosphoglycerate Kinase
Polyploidy
Spectrophotometry
Thymidine Kinase
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DOI
https://doi.org/10.1007/bf00487007
TNO identifier
227340
ISSN
0006-2928
Source
Biochemical Genetics, 7 (7), 33-40
Document type
article