Title
The small heat-shock protein αB-crystallin as candidate autoantigen in multiple sclerosis
Author
van Noort, J.M.
van Sechel, A.C.
Bajramovic, J.J.
El Quagmiri, M.
Polman, C.H.
Lassmann, H.
Ravid, R.
TNO Preventie en Gezondheid
Publication year
1995
Abstract
The identification of key antigens in human autoimmune diseases is a crucial step towards the development of specific intervention. The autoantigen(s) relevant to multiple sclerosis (MS) probably reside in myelin of the central nervous system, the target of the disease. Here we examine proliferative responses of human peripheral blood T cells to the complete collection of myelin proteins fractionated by reversed-phase high-performance liquid chromatography. Myelin isolated from MS-affected brain contained a single protein fraction to which T cells from MS patients and from healthy controls showed dominant responses. This highly immunogenic protein was identified as αB-crystallin, a small heat-shock protein. Immunohistochemical examination of MS lesions revealed the presence of oligodendrocytes and astrocytes with raised αB-crystallin expression, which were not found in unaffected myelin. Our findings indicate that αB-crystallin serves as immunodominant myelin antigen to human T cells when expressed at the elevated levels found in active MS lesions. Chemicals/CAS: crystallin, 11046-99-4; Autoantigens; Crystallins; Heat-Shock Proteins; Myelin Proteins
Subject
Biology
Astrocytes
Autoantigens
Cells, Cultured
Crystallins
Heat-Shock Proteins
Human
Lymphocyte Activation
Multiple Sclerosis
Myelin Proteins
Myelin Sheath
Oligodendroglia
T-Lymphocytes
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TNO identifier
232924
ISSN
0028-0836
Source
Nature, 375 (6534), 798-801
Document type
article