Title
The chicken enucleated eye test (CEET): A practical (pre)screen for the assessment of eye irritation/corrosion potential of test materials
Author
Prinsen, M.K.
Instituut CIVO-Toxicologie en Voeding TNO
Publication year
1996
Abstract
The enucleated eye test with chicken eyes (CEET) obtained from an abattoir proved to be a valuable and practical alternative for the 'traditional' enucleated eye test with eyes of laboratory rabbits. Since 1992, the CEET has been incorporated in standard contract toxicity testing at the Toxicology Division of the TNO Nutrition and Food Research Institute as a (pre)screen for the Draize eye test with rabbits. The results of the first 44 compounds tested showed excellent correlation with the in vivo results. The CEET identified non-irritating or severely irritating compounds, and predicted (slightly to moderately) irritating compounds. Statistical analysis of the CEET and the rabbit in vivo scores showed high linear correlations between the critical values of both tests and confirmed the relevance of this assay with respect to ocular effects. In general, tiered in vitro/in vivo testing was considered a meaningful approach for further validation of alternative methods and for reducing the use of suffering of laboratory animals to a minimum. Tiered testing of compounds in cases of eye irritation hazard assessment should be incorporated in the legislation of the European Community. Chemicals/CAS: Irritants
Subject
Toxicology
Animal Testing Alternatives
Animals
Chickens
Cornea
Corneal Opacity
Drug Evaluation, Preclinical
Epithelium
Eye Enucleation
Irritants
Rabbits
Toxicity Tests
Animalia
Gallus gallus
Oryctolagus cuniculus
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DOI
https://doi.org/10.1016/0278-6915(95)00115-8
TNO identifier
68396
ISSN
0278-6915
Source
Food and Chemical Toxicology, 34 (3), 291-296
Document type
article