Title
Pericellular-acting proteases in human first trimester decidua
Author
Plaisier, M.
Koolwijk, P.
Willems, F.
Helmerhorst, F.M.
van Hinsbergh, V.W.M.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
Proteolysis is essential for decidual development during embryonic implantation, but little is known regarding the expression and functions of membrane-type matrix metalloproteinases (MT-MMPs) and urokinase-type plasminogen activator (uPA) and its receptor uPAR in decidua. Therefore, their protein and mRNA levels were analysed in three first trimester decidual tissues, decidual secretory endometrium (DSE), decidua parietalis (DP) and basalis (DB). Decidua was obtained during first trimester pregnancy termination. uPA, uPAR, and MT1/2/3/5-MMP expression were studied by RT-PCR and immunohistochemistry, and CD56-positive uNK cells and CD68-positive macrophages were quantified in serial sections. The mRNAs and antigens of all proteases and uPAR were detectable in the decidual tissues and extravillous trophoblasts (EVT). mRNA levels of all proteases and uPAR, except MT5-MMP, were elevated in both DB and DP compared to DSE, being significant for MT1-MMP and uPAR in DP. MT2- and MT3-MMP mRNAs in DB were 24- and 10-fold higher than in DSE, and 19- and 7-fold increased compared to DP. At the protein level uPA and uPAR were particularly elevated in DB, while pro-angiogenic MT1- and MT3-MMPs were elevated in both DB and DP compared to DSE. MT2-MMP was prominently present in all conditions. The number of uNK cells was increased in DB and DP versus DSE, while a comparable increase in macrophages did not reach statistical significance. These data are consistent with a differential regulation of pericellular proteases in decidua by pregnancy-induced hormones, immune cells and EVT. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Subject
Healthy for Life
Biology
Healthy Living
Biomedical Research
Decidua
First trimester
Matrix metalloproteinases
Trophoblast
UNK cells
CD56 antigen
CD68 antigen
matrix metalloproteinase
messenger RNA
proteinase
urokinase
urokinase receptor
adult
article
cell count
controlled study
decidua
female
first trimester pregnancy
gene expression
human
human cell
human tissue
immunohistochemistry
macrophage
natural killer cell
pathophysiology
pregnancy termination
priority journal
protein analysis
protein degradation
protein expression
protein function
quantitative analysis
reverse transcription polymerase chain reaction
spontaneous abortion
trophoblast
Adult
Antigens, CD
Antigens, CD56
Antigens, Differentiation, Myelomonocytic
Decidua
Female
Humans
Immunohistochemistry
Matrix Metalloproteinase 14
Matrix Metalloproteinase 20
Peptide Hydrolases
Pregnancy
Pregnancy Trimester, First
Reverse Transcriptase Polymerase Chain Reaction
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http://resolver.tudelft.nl/uuid:fccd046e-334d-48e5-a52a-fc7961c703a9
DOI
https://doi.org/10.1093/molehr/gam085
TNO identifier
240590
ISSN
1360-9947
Source
Molecular Human Reproduction, 14 (1), 41-51
Document type
article