Print Email Facebook Twitter Nutrient-induced glucagon like peptide-1 release is modulated by serotonin Title Nutrient-induced glucagon like peptide-1 release is modulated by serotonin Author Ripken, D. van der Wielen, N. Wortelboer, H.M. Meijerink, J. Witkamp, R.F. Hendriks, H.F.J. Publication year 2016 Abstract Glucagon like peptide-1 (GLP-1) and serotonin are both involved in food intake regulation. GLP-1 release is stimulated upon nutrient interaction with G-protein coupled receptors by enteroendocrine cells (EEC), whereas serotonin is released from enterochromaffin cells (ECC). The central hypothesis for the current study was that nutrient-induced GLP-1 release from EECs is modulated by serotonin through a process involving serotonin receptor interaction. This was studied by assessing the effects of serotonin reuptake inhibition by fluoxetine on nutrient-induced GLP-1, PYY and CCK release from isolated pig intestinal segments. Next, serotonin-induced GLP-1 release was studied in enteroendocrine STC-1 cells, where effects of serotonin receptor inhibition were studied using specific and non-specific antagonists.Casein (1% w/v), safflower oil (3.35% w/v), sucrose (50 mM) and rebaudioside A (12.5 mM) stimulated GLP-1 release from intestinal segments, whereas casein only stimulated PYY and CCK release. Combining nutrients with fluoxetine further increased nutrient-induced GLP-1, PYY and CCK release.Serotonin release from intestinal tissue segments was stimulated by casein and safflower oil while sucrose and rebaudioside A had no effect. The combination with fluoxetine (0.155 μM) further enhanced casein and safflower oil induced-serotonin release.Exposure of ileal tissue segments to serotonin (30 μM) stimulated GLP-1 release whereas it did not induce PYY and CCK release. Serotonin (30 and 100 μM) also stimulated GLP-1 release from STC-1 cells, which was inhibited by the non-specific 5HT receptor antagonist asenapine (1 and 10 μM). These data suggest that nutrient-induced GLP-1 release is modulated by serotonin through a receptor mediated process. © 2016 The Authors. Subject LifeMSB - Microbiology and Systems BiologyELSS - Earth, Life and Social SciencesBiomedical InnovationBiologyHealthy LivingGLP-1NutrientsRebaudioside ASerotoninSmall intestine To reference this document use: http://resolver.tudelft.nl/uuid:fca95616-942c-49f6-a365-99051e96b86c DOI https://doi.org/10.1016/j.jnutbio.2016.03.006 TNO identifier 534888 ISSN 0955-2863 Source Journal of Nutritional Biochemistry, 32, 142-150 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.