Title
The potential of Lactobacillus as a carrier for oral immunization: Development and preliminary characterization of vector systems for targeted delivery of antigens
Author
Centraal Instituut voor Voedingsonderzoek TNO TNO Preventie en Gezondheid
Pouwels, P.H.
Leer, R.J.
Boersma, W.J.A.
Publication year
1996
Abstract
Oral administration of lactobacilli evokes mucosal and systemic immune responses against epitopes associated with these organisms (Gerritse et al., 1990, 1991). The adjuvant function of different Lactobacillus species was investigated under the conditions of intraperitoneal (i.p.) injection or oral administration. After i.p. injection of trinitrophenylated chicken γ-globulin, high DTH responses were observed with Lactobacillus casei and Lactobacillus plantarum, but low responses with Lactobacillus fermentum and Lactobacillus delbrueckii subsp. bulgaricus. In different experimental model systems L. casei and L. plantarum consistently showed significant adjuvanticity. A series of expression and expression-secretion vectors containing the strong constitutive promoter of the L. casei L-ldh gene or the regulatable promoter of the Lactobacillus amylovorus amy gene (Pouwels and Leer, 1995) was used for the intracellular, extracellular and surface-bound expression of an influenza virus antigenic determinant fused to Escherichia coli,B-glucuronidase. Intracellular expression of the fusion protein amounted to 1-2% of total soluble protein. Lactobacilli synthesizing the fusion protein intracellularly evoked an oral immune response after subcutaneous priming. Oral administration of lactobacilli evokes mucosal and systemic immune responses against epitopes associated with these organisms. The adjuvant function of different Lactobacillus species was investigated under the conditions of intraperitoneal (i.p.) injection or oral administration. After i.p. injection of trinitrophenylated chicken γ-globulin, high DTH responses were observed with Lactobacillus casei and Lactobacillus plantarum, but low responses with Lactobacillus fermentum and Lactobacillus delbrueckii subsp. bulgaricus. In different experimental model systems L. casei and L. plantarum consistently showed significant adjuvanticity. A series of expression and expression-secretion vectors containing the strong constitutive promoter of the L. casei L-ldh gene or the regulatable promoter of the Lactobacillus amylovorus amy gene was used for the intracellular, extracellular and surface-bound expression of an influenza virus antigenic determinant fused to Escherichia coli β-glucuronidase. Intracellular expression of the fusion protein amounted to 1-2% of total soluble protein. Lactobacilli synthesizing the fusion protein intracellularly evoked an oral immune response after subcutaneous priming. Chemicals/CAS: Bacterial Vaccines; beta-Galactosidase, EC 3.2.1.23; Epitopes; Glucuronidase, EC 3.2.1.31
Subject
Adjuvant
Expression vector
Influenza
Lactobacillus
Oral immunization
Antigens
Coliform bacteria
Disease control
Diseases
Drug therapy
Genes
Proteins
Vaccines
Viruses
Adjuvants
Epitopes
Expression vectors
Influenza
Lactobacillus
Oral immunization
Immunization
Adjuvant
Bacterial vaccine
Animal experiment
Animal model
Conference paper
Controlled study
Expression vector
Influenza
Intraperitoneal drug administration
Lactobacillus
Mouse
Nonhuman
Oral drug administration
Priority journal
Vaccination
Administration, Oral
Animals
Antibody Formation
Bacterial Vaccines
beta-Galactosidase
Epitopes
Gene Expression
Genes, Bacterial
Glucuronidase
Hypersensitivity, Delayed
Immunization
Injections, Intraperitoneal
Lactobacillus
Lactobacillus acidophilus
Lactobacillus casei
Mice
Plasmids
Restriction Mapping
Species Specificity
Animalia
Bacteria (microorganisms)
Escherichia
Escherichia coli B
Gallus gallus
Influenza virus
lactobacilli casei
Lactobacillus
Lactobacillus amylovorus
Lactobacillus casei
Lactobacillus delbrueckii subsp. bulgaricus
Lactobacillus fermentum
Lactobacillus plantarum
To reference this document use:
http://resolver.tudelft.nl/uuid:f73967ce-2d2c-401f-94f6-3b7941e78ccb
DOI
https://doi.org/10.1016/0168-1656(95)00140-9
TNO identifier
233198
ISSN
0168-1656
Source
Journal of Biotechnology, 44 (44), 183-192
Document type
article