Print Email Facebook Twitter Effect of experimental gingivitis induction and erythritol on the salivary metabolome and functional biochemistry of systemically healthy young adults Title Effect of experimental gingivitis induction and erythritol on the salivary metabolome and functional biochemistry of systemically healthy young adults Author Prodan, A. Imangaliyev, S. Brand, H.S. Rosema, M.N.A. Levin, E. Crielaard, W. Keijser, B.J.F. Veerman, E.C.I. Publication year 2016 Abstract Introduction: Understanding the changes occurring in the oral ecosystem during development of gingivitis could help improve prevention and treatment strategies for oral health. Erythritol is a non-caloric polyol proposed to have beneficial effects on oral health. Objectives: To examine the effect of experimental gingivitis and the effect of erythritol on the salivary metabolome and salivary functional biochemistry. Methods: In a two-week experimental gingivitis challenge intervention study, non-targeted, mass spectrometry-based metabolomic profiling was performed on saliva samples from 61 healthy adults, collected at five time-points. The effect of erythritol was studied in a randomized, controlled trial setting. Fourteen salivary biochemistry variables were measured with antibody- or enzymatic activity-based assays. Results: Bacterial amino acid catabolites (cadaverine, N-acetylcadaverine, and a-hydroxyisovalerate) and end-products of bacterial alkali-producing pathways (N-a-acetylornithine and ?-aminobutyrate) increased significantly during the experimental gingivitis. Significant changes were found in a set of 13 salivary metabolite ratios composed of host cell membrane lipids involved in cell signaling, host responses to bacteria, and defense against free radicals. An increase in mevalonate was also observed. There were no significant effects of erythritol. No significant changes were found in functional salivary biochemistry. Conclusions: The findings underline a dynamic interaction between the host and the oral microbial biofilm during an experimental induction of gingivitis. © 2016, Springer Science+Business Media New York. Subject LifeMSB - Microbiology and Systems BiologyELSS - Earth, Life and Social SciencesBiomedical InnovationBiologyHealthy LivingChallenge interventionErythritolExperimental gingivitisMetabolomicsOral healthSaliva To reference this document use: http://resolver.tudelft.nl/uuid:f6a98e8a-8b07-49a9-b17d-5eea4ef3a0ce DOI https://doi.org/10.1007/s11306-016-1096-4 TNO identifier 572341 ISSN 1573-3882 Source Metabolomics, 12 (10) Article number 147 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.