Print Email Facebook Twitter Comparison of five incubation systems for rat liver slices using functional and viability parameters Title Comparison of five incubation systems for rat liver slices using functional and viability parameters Author Olinga, P. Groen, K. Hof, I.H. de Kanter, R. Koster, H.J. Leeman, W.R. Rutten, A.A.J.J.L. van Twillert, K. Groothuis, G.M.M. Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding Publication year 1997 Abstract Precision-cut liver slices are presently used for various research objects, e.g. to study metabolism, transport, and toxicity of xenobiotics. Various incubation systems are presently employed, but a systematic comparison between these incubation systems with respect to preservation of slice function has not been performed yet. Therefore, we started a comparative study to evaluate five of these systems: the shaken flask (an Erlenmeyer in a shaking water bath), the stirred-well (24-well culture plate equipped with grids and magnetic stirrers), rocker platform (6-well culture plate with Netwell insert rocked on a platform), the roller system (dynamic organ culture rolled on an insert in a glass vial), and the 6-well shaker (6-well culture plate in a shaking water bath). The liver slices were incubated in these incubation systems for 0.5, 1.5, and 24.5 h and subsequently subjected to viability and metabolic function tests. The viability of the incubated liver slices was evaluated by: potassium content, MTT assay, energy charge, histomorphology, and LDH leakage. Their metabolic functions were studied by determination of the metabolism of lidocaine, testosterone, and antipyrine. Up to 1.5 h of incubation all five incubation systems gave similar results with respect to viability and metabolic function of the liver slices. However, after 24 h, the shaken flask, the rocker platform, and the 6-well shaker incubation systems appeared to be superior to the stirred well and the roller incubation systems. Chemicals/CAS: L-Lactate Dehydrogenase, EC 22.214.171.124; Potassium, 7440-09-7; Xenobiotics Subject Incubation systemRat liver slicesVaibilityLidocainePhenazoneTestosteroneAnimal tissueCell viabilityControlled studyCulture mediumDrug metabolismIncubation timeLiver microsome metabolismLiver sliceNonhumanPreservationAnimalsEnergy MetabolismL-Lactate DehydrogenaseLiverMaleOrgan Culture TechniquesPotassiumRatsRats, WistarXenobioticsAnimalia To reference this document use: http://resolver.tudelft.nl/uuid:f6794b84-6a11-4b01-a6c0-e932d2d5367e DOI https://doi.org/10.1016/s1056-8719(97)00060-9 TNO identifier 234070 ISSN 1056-8719 Source Journal of Pharmacological and Toxicological Methods, 38 (2), 59-69 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.