Title
Allergen Ara h 1 occurs in peanuts as a large oligomer rather than as a trimer
Author
van Boxtel, E.L.
van Beers, M.M.C.
Koppelman, S.J.
van den Broek, L.A.M.
Gruppen, H.
TNO Kwaliteit van Leven
Publication year
2006
Abstract
Ara h 1, a major peanut allergen, is known as a stable trimeric protein. Nevertheless, upon purification of native Ara h 1 from peanuts using only size exclusion chromatography, the allergen appeared to exist in an oligomeric structure, rather than as a trimeric structure. The oligomeric structure was independent of the salt concentration applied. Subjecting the allergen to anion exchange chromatography induced the allergen to dissociate into trimers. Ammonium sulfate precipitation did not bring about any structural changes, whereas exposing the allergen to hydrophobic interaction chromatography caused it to partly dissociate into trimers, with increasing amounts of trimers at higher ionic strengths. The (partial) dissociation into trimers led to a change in the tertiary structure of the monomeric subunits of the allergen, with the monomers in Ara h 1 oligomers having a more compact tertiary structure compared with the monomers in Ara h 1 trimers. As structural characteristics are important for a protein's allergenicity, this finding may imply a different allergenicity for Ara h 1 than previously described. © 2006 American Chemical Society.
Subject
Nutrition
Food technology
Ara h 1
Food allergen
Peanut
Protein structure
allergen
ammonium sulfate
Ara h 1 protein, Arachis hypogaea
glycoprotein
vegetable protein
article
chemistry
gel chromatography
osmolarity
peanut
plant seed
polyacrylamide gel electrophoresis
precipitation
protein quaternary structure
Allergens
Ammonium Sulfate
Arachis hypogaea
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Glycoproteins
Osmolar Concentration
Plant Proteins
Precipitation
Protein Structure, Quaternary
Seeds
Ara
Arachis hypogaea
To reference this document use:
http://resolver.tudelft.nl/uuid:f5ff702b-b2db-4940-b6ec-26fb9725b464
DOI
https://doi.org/10.1021/jf061433+
TNO identifier
239482
ISSN
0021-8561
Source
Journal of Agricultural and Food Chemistry, 54 (19), 7180-7186
Document type
article