Title
Expression of human lymphotoxin alpha in Aspergillus niger
Author
Krasevec, N.
van de Hondel, C.A.M.J.J.
Komel, R.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2000
Abstract
A gene-fusion expression strategy was applied for heterologous expression of human lymphotoxin alpha (LTα) in the Aspergillus niger AB1.13 protease-deficient strain. The LTα gene was fused with the A. niger glucoamylase GII-form as a carrier-gene, behind its transcription control and secretion signals. Special attention was paid to the influence of different codon usage on secretion of protein. In the case of human tumor necrosis factor alpha (TNFα) a dramatic change of secretion has been observed when human cDNA sequence was used instead of synthetic E. coli biased codons. In the case of LTα such a change of codon usage brought improvement at the RNA level, however, no increase in the quantity of secreted protein was observed, due to the proteolitic activity of the host organism. The estimated yield of secretion of LTα from A. niger into the soya medium was 50 pg l-1 of culture. Chemicals/CAS: Glucan 1,4-alpha-Glucosidase, EC 3.2.1.3; Lymphotoxin-alpha; RNA, Messenger
Subject
Medicine
Geneeskunde
Pathology
Aspergillus niger
Codon usage
Heterologous protein secretion
LTα
TNFα
Complementary DNA
Lymphotoxin
Tumor necrosis factor alpha
Aspergillus niger
Codon usage
Conference paper
Controlled study
DNA sequence
Gene control
Gene fusion
Genetic transcription
Human
Nonhuman
Priority journal
Protein degradation
Protein secretion
Artificial Gene Fusion
Aspergillus niger
Blotting, Northern
Glucan 1,4-alpha-Glucosidase
Humans
Lymphotoxin-alpha
RNA, Messenger
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TNO identifier
56828
ISSN
0031-6768
Source
Pflügers Archiv - European Journal of Physiology, 440 (suppl. 5), p. R83-R85
Document type
article