Title
CETP does not affect triglyceride production or clearance in APOE*3-Leiden mice
Author
TNO Kwaliteit van Leven
Bijland, S.
van den Berg, S.A.A.
Voshol, P.J.
van den Hoek, A.M.
Princen, H.M.G.
Havekes, L.M.
Rensen, P.C.N.
van Dijk, K.W.
Publication year
2010
Abstract
The cholesteryl ester transfer protein (CETP) facilitates the bidirectional transfer of cholesteryl esters and triglycerides (TG) between HDL and (V)LDL. By shifting cholesterol in plasma from HDL to (V)LDL in exchange for VLDL-TG, CETP aggravates atherosclerosis in hyperlipidemic APOE*3-Leiden (E3L) mice. The aim of this study was to investigate the role of CETP in TG metabolism and high-fat diet-induced obesity by using E3L mice with and without the expression of the human CETP gene. On chow, plasma lipid levels were comparable between both male and female E3L and E3L. CETP mice. Further mechanistic studies were performed using male mice. CETP expression increased the level of TG in HDL. CETP did not affect the postprandial plasma TG response or the hepatic VLDL-TG and VLDL-apolipoprotein B production rate. Moreover, CETP did not affect the plasma TG clearance rate or organ-specific TG uptake after infusion of VLDL-like emulsion particles. In line with the absence of an effect of CETP on tissue-specific TG uptake, CETP also did not affect weight gain in response to a high-fat diet. In conclusion, the CETP-induced increase of TG in the HDL fraction of E3L mice is not associated with changes in the production of TG or with tissue-specific clearance of TG from the plasma. Copyright © 2010 by the American Society for Biochemistry and Molecular Biology, Inc.
Subject
Food and Chemical Risk Analysis
Cholesteryl ester transfer protein
HDL
Postprandial lipoproteins
Triglyceride metabolism
To reference this document use:
http://resolver.tudelft.nl/uuid:f36a8387-86cb-4ba0-814a-b504cc66dfc4
TNO identifier
273918
ISSN
0022-2275
Source
Journal of Lipid Research, 51 (51), 97-102
Document type
article