Title
Assignment of the human gene for histidine-rich glycoprotein to chromosome 3
Author
Gaubius instituut TNO
van den Berg, E.A.
Le Clercq, E.
Kluft, C.
Koide, T.
van der Zee, A.
Oldenburg, M.
Wijnen TH., J.
Meera Khan, P.
Publication year
1990
Abstract
Histidine-rich glycoprotein (HRG) is a monomeric plasma glycoprotein involved in the modulation of coagulation and fibrinolysis. Using Southern analysis of human-rodent somatic cell hybrid DNA with a human HRG-specific cDNA probe, the HRG gene was assigned to chromosome 3. One hybrid that was known to contain only a segment of chromosome 3 also reacted positively with the HRG probe. Hybridization analysis with a set of chromosome 3-specific probes showed that the segment of chromosome 3 present in this hybrid is missing the region pter-p14, which indicates that HRG is not located in this region. No restriction fragment length polymorphisms were detected for HRG with 10 commonly used restriction enzymes. Chemicals/CAS: Glycoproteins; histidine-rich proteins; Proteins
Subject
histidine rich glycoprotein
unclassified drug
article
chromosome 3
gene assignment
human
human cell
Animal
Blotting, Southern
Chromosome Mapping
Chromosomes, Human, Pair 3
Genes
Glycoproteins
Human
Hybrid Cells
Proteins
Rodentia
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DOI
https://doi.org/10.1016/0888-7543(90)90551-5
TNO identifier
231195
ISSN
0888-7543
Source
Genomics, 7 (7), 276-279
Document type
article