Title
Allelic diversity at the Mhc-DP locus in rhesus macaques (Macaca mulatta)
Author
Primatencentrum TNO
Slierendregt, B.L.
Otting, N.
Kenter, M.
Bontrop, R.E.
Publication year
1995
Abstract
Allelic diversity at the major histocompatibility complex class II DP locus of rhesus macaques was studied by sequencing exon 2 of Mamu-DPA1 and -DPB1 genes. The Mamu-DPA1 gene is apparently invariant, whereas the Mamu-DPB1 locus displays polymorphism. Here we report the characterization of 1 Mamu-DPA1 and 13 Mamu-DPB1 alleles which were compared with other available primate Mhc-DPA1 and -DPB1 sequences. As compared with Mhc-DRB and -DQB1, most codons for the contact residues in the antigen binding site of the primate Mhc-DPB1 gene have a relatively low degree of variation in encoding various types of amino acids. In contrast to Mhc-DRB and -DQB, the HLA- and Mamu-DPB1 sequences cluster in a species-specific manner in phylogenetic trees. Mhc-DPB1 polymorphisms, however, are inherited in a transspecies mode of evolution, as is demonstrated by the sharing of lineage members between closely related macaque species. The data demonstrate that the transspecies character of Mhc-DPB1 polymorphism was retained over much shorter periods of time as compared with its sister class II loci, Mhc-DQ and -DR.
Subject
HLA DP antigen
HLA DQ antigen
HLA DR antigen
Animal cell
Antibody combining site
Biodiversity
Codon
Comparative study
Exon
Gene locus
Genetic polymorphism
Macaca
Major histocompatibility complex
Nonhuman
Phylogeny
Rhesus monkey
Alleles
Amino acid sequence
Animal
Base sequence
DNA probes
Genes, MHC Class II
Macaca mulatta
Molecular sequence data
Phylogeny
Polymerase chain reaction
Sequence alignment
Animalia
Macaca
Primates
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DOI
https://doi.org/10.1007/bf00188429
TNO identifier
232853
ISSN
0093-7711
Source
Immunogenetics, 41 (41), 29-37
Document type
article