Title
Food allergen (peanut)-specific TH2 clones generated from the peripheral blood of a patient with peanut allergy
Author
de Jong, E.C.
Spanhaak, S.
Martens, B.P.M.
Kapsenberg, M.L.
Penninks, A.H.
Wierenga, E.A.
TNO Voeding
Publication year
1996
Abstract
Background: Increasing evidence indicates a prominent role of allergen-specific TH2 cells, with high IL-4 and IL-5 production and low interferon-γ production, in the regulation of IgE and eosinophil production in allergic disorders. However, most studies have concentrated on T cells reactive with inhalation allergens, whereas little is known about the properties of food allergen-reactive T cells. Objective: In this study we therefore characterized peanut-specific T cells, cloned from a patient with severe peanut allergy. Methods: Peripheral blood mononudear cells from patients with peanut allergy and nonallergic individuals were stimulated with crude peanut extract (CPE) to compare the proliferative responses and to select a suitable patient for the cloning of CPE-specific T cells. The resultant panel of CPE-reactive T-lymphocyte clones was serologically phenotyped by flow cytometty and analyzed for cytokine secretion by ELISA. Results: The patients' peripheral blood mononudear cells showed a dose-dependent proliferative response to CPE, which was significantly higher (p < 0.05) than in peripheral blood mononudear cells of nonallergic donors. The CPE-specific T-lymphocyte clones generated from the selected patient were all CD4+/CD8- T helper cells with a TH2 cytokine profile, secreting high amounts of IL-4 and IL-5, but little or no interferon-γ. Conclusions: This study demonstrates that peanut-specific T cells do occur in the peripheral blood of patients with peanut allergy and suggests an increased frequency of these T cells in patients compared with nonallergic control subjects. The CD4+ phenotype and the TH2 cytokine profile of the CPE-specific T-lymphocyte clones suggest a functional role of allergen-specific TH2 cells in the pathophysiology of food allergy, similar to the function of inhalation allergen-specific TH2 cells. Copyright © 1996 by Mosby-Year Book, Inc.
Subject
Toxicology
Food allergy
Peanut
Phenotype
T-cell clones
Food allergen
Immunoglobulin e
Interleukin 4
Interleukin 5
Adolescent
Case report
Cell clone
Controlled study
Helper cell
T lymphocyte subpopulation
Adult
Antigens, Surface
Arachis hypogaea
Clone Cells
Cytokines
Epitopes
Female
Food Hypersensitivity
Humans
Lymphocyte Activation
Male
Plant Proteins
Th2 Cells
To reference this document use:
http://resolver.tudelft.nl/uuid:f1151cd5-6a06-4005-9e27-2802bbf4c49b
TNO identifier
233589
ISSN
0091-6749
Source
Journal of Allergy and Clinical Immunology, 98 (1), 73-81
Document type
article