Title
Effect of urinary pH on the progression of urinary bladder tumours
Author
Lina, B.A.R.
van Garderen-Hoetmer, A.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
Systemic alkalosis has been postulated to enhance tumorigenesis, whereas systemic acidosis has been implicated to exert a favourable influence on tumour control and regression. In the present study the urinary pH was influenced by feeding acid-forming or base-forming diets, and the effect of alkaline or acid urine on the early and late progression phase of urinary bladder carcinogenicity was investigated in male Wistar rats. Bladder lesions were initiated by N-butyl-N-(4-hydroxybutyl) nitrosamine (0.05% BBN in the drinking water during 4 weeks) and promoted by sodium bicarbonate (3.4% NaHCO3 in the diet during 15 or 25 weeks). After short- (15 week) and more long-term (25 week) promotion with NaHCO3, groups of 20 rats were fed a diet containing the acidifying salt ammoniumchloride (2.1% NH4Cl) or the control diet. All surviving rats were killed after a total study duration of 52 weeks. Additional control groups were, after initiation, fed diets containing NaHCO3 and killed after 15 wk or 25 wk of promotion, or at the end of the study. In rats fed diets with added salts, water intake and the amount of urine produced were increased and the urinary density was decreased compared to rats fed control diet. During NaHCO3 feeding, urinary pH and sodium concentration were increased. During NH4Cl feeding, urinary pH was decreased and urinary chloride and calcium concentrations were increased. Initiation by BBN followed by treatment with NaHCO3 caused a high incidence of papillary/nodular hyperplasia, papillomas and carcinomas of the bladder epithelium. These lesions progressed with time or longer duration of NaHCO3 promotion. A tumour protective effect of urinary acidification by NH4Cl was not found. In fact, both acidification and prolonged alkalinization tended to aggravate the malignancy of bladder carcinomas. Copyright (C) 2000 Elsevier Science Ltd.
Subject
Nutrition
Acidosis
Alkalosis
Progression
Urinary bladder tumours
Ammonium chloride
Bicarbonate
Calcium
Chloride
N butyl n (4 hydroxybutyl)nitrosamine
Sodium
Acidosis
Alkalosis
Animal experiment
Animal tissue
Bladder carcinogenesis
Bladder carcinoma
Bladder injury
Bladder papilloma
Bladder tumor
Calcium urine level
Chloride urine level
Controlled study
Diet
Fluid intake
Male
Nonhuman
Rat
Sodium urine level
Tumor growth
Urine pH
Urine volume
Animals
Disease Progression
Hydrogen-Ion Concentration
Male
Rats
Rats, Wistar
Urinary Bladder Neoplasms
Rattus norvegicus
To reference this document use:
http://resolver.tudelft.nl/uuid:f0d4de9b-7135-42f1-8274-70d89b976b40
DOI
https://doi.org/10.1016/s0278-6915(99)00111-8
TNO identifier
41353
ISSN
0278-6915
Source
Food and Chemical Toxicology, 37 (12), 1159-1166
Document type
article