Print Email Facebook Twitter Effect of fortified spread on homocysteine concentration in apparently healthy volunteers Title Effect of fortified spread on homocysteine concentration in apparently healthy volunteers Author van Vliet, T. Jacobs, R.G.J.M. de Deckere, E. van den Berg, H. de Bree, A. van der Put, N.M.J. TNO Kwaliteit van Leven Publication year 2007 Abstract Objective: To determine the effect of folic acid, vitamin B6 and B12 fortified spreads on the blood concentrations of these vitamins and homocysteine. Design and setting: A 6-week randomized, double-blinded, placebo-controlled, parallel trial carried out in a clinical research center. Subjects: One hundred and fifty healthy volunteers (50% males). Interventions: For 6 weeks, the subjects consumed the test spreads (20 g/day): containing per 20 g (1) 200μ g folic acid, 2μ g vitamin B12 and 1 mg vitamin B6, or (2) 400μ g folic acid, 2μ g vitamin B12 and 1 mg vitamin B6 or (3) no B-vitamins (control spread). Results: The B-vitamin status increased on using the test spreads, with the largest effect on the serum folate concentration: 48% in men and 58% in women on spread 1 and 92 and 146%, respectively, on spread 2 (P-values all <0.05). The plasma homocysteine decreased in the groups treated with the fortified spreads as compared to the control group. Average decreases were for males: 0.7±1.5±μmol/l (6.8%) on spread 1 and 1.7 ± 1.7 μmol/l (17.6%) on spread 2 and for females: 1.4 ± 1.2 μmol/l (14.2%) and 2.4 ± 2.0 μmol/l (23.3%), respectively (P-values all <0.05). Conclusions: Consumption of a spread fortified with folic acid, vitamin B6 and vitamin B12 for 6 weeks significantly increases the blood concentrations of these vitamins and significantly decreases the plasma concentration of homocysteine. Fortified staple foods like spreads can contribute to the lowering of homocysteine concentrations. Subject HealthBiomedical Researchcyanocobalaminfolic acidhomocysteinepyridoxinevitamin B complexadultagedamino acid blood levelarticleclinical researchclinical trialcontrolled studydouble blind procedurefemalefolic acid blood levelfood intakehumanmalenormal humanparallel designrandomized controlled trialvitamin blood levelvitamin supplementationvolunteeradolescentbloodcontrolled clinical trialdiet supplementationdiet therapydose responsehyperhomocysteinemiamiddle agedsex differenceAdolescentAdultAgedDose-Response Relationship, DrugDouble-Blind MethodFemaleFolic AcidFood, FortifiedHomocysteineHumansHyperhomocysteinemiaMaleMiddle AgedSex FactorsVitamin B 12Vitamin B 6Vitamin B Complex To reference this document use: http://resolver.tudelft.nl/uuid:f05c3f14-b855-4022-beb3-abe239f14841 DOI https://doi.org/10.1038/sj.ejcn.1602570 TNO identifier 240013 ISSN 0954-3007 Source European Journal of Clinical Nutrition, 61 (6), 769-778 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.