Title
Immunization with mannosylated peptide induces poor T cell effector functions despite enhanced antigen presentation
Author
Kel, J.M.
de Geus, E.D.
van Stipdonk, M.J.
Drijfhout, J.W.
Koning, F.
Nagelkerken, L.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
In this study, we investigated the development of T cell responses in mice after administration of a mannosylated ovalbumin peptide (M-OVA323-339). Immunization with M-OVA323-339 in complete adjuvant resulted in enhanced antigen presentation in draining lymph nodes. Monitoring the fate of CFSE-labeled ovalbumin peptide-specific TCR transgenic CD4+ T cells revealed that immunization with M-OVA323-339 induced normal clonal expansion, recirculation and CD62L expression of antigen-specific T cells in vivo. However, these T cells developed only poor effector functions, reflected by minimal IFN-γ production, low IgG2a levels in serum and poor peptide-specific delayed-type hypersensitivity (DTH) responses. This diminished inflammatory response was associated with decreased infiltration of T cell blasts and macrophages. Importantly, also mice with functional effector T cells did not mount a robust DTH response after a challenge with M-OVA323-339 in the ear, although their T cells responded normally to M-OVA323-339 in vitro. In conclusion, mannosylated peptide induces proliferation of T cells with impaired Th1 cell effector functions and additionally abrogates the activity of pre-existing effector T cells. © The Japanese Society for Immunology. 2007. All rights reserved.
Subject
C-type lectins
Delayed-type hypersensitivity
Immune modulation
Th1 immunity
CD4 antigen
gamma interferon
immunoglobulin G2a
L selectin
ovalbumin
T lymphocyte receptor
animal cell
animal experiment
animal tissue
antigen expression
antigen presentation
antigen specificity
antiinflammatory activity
article
CD4+ T lymphocyte
cell fate
cellular immunity
clonal variation
controlled study
cytokine production
delayed hypersensitivity
effector cell
female
immunization
immunoglobulin blood level
in vitro study
in vivo study
lymph node
lymphoblast
lymphocyte proliferation
lymphocytic infiltration
macrophage
molecular recognition
mouse
nonhuman
priority journal
spleen cell
T lymphocyte
Th1 cell
Amino Acid Sequence
Animals
Antigen Presentation
Female
Hypersensitivity, Delayed
Immunization
Lymphocyte Activation
Mannose
Mice
Mice, Inbred C57BL
Mice, Transgenic
Molecular Sequence Data
Ovalbumin
Peptides
Receptors, Antigen, T-Cell
Th1 Cells
To reference this document use:
http://resolver.tudelft.nl/uuid:efad7855-ffa4-4817-ac71-fb69cc13ee1e
DOI
https://doi.org/10.1093/intimm/dxm123
TNO identifier
240594
ISSN
0953-8178
Source
International Immunology, 20 (1), 117-127
Document type
article