Title
Methyl bromide causes DNA methylation in rats and mice but fails to induce somatic mutations in λlacZ transgenic mice
Author
Pletsa, V.
Steenwinkel, M.-J.S.T.
van Delft, J.H.M.
Baan, R.A.
Kyrtopoulos, S.A.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1998
Abstract
Following single or multiple oral treatments of rats or λlacZ transgenic mice with methyl bromide, methylated DNA adducts (N7- and/or O6-methylguanine) were found at comparable levels in various tissues, including among others the glandular stomach, the forestomach and the liver. Multiple rat treatment resulted in substantial decreases in the repair enzyme O6-alkylguanine-DNA alkyltransferase which were probably due in part to direct interaction of the enzyme with methyl bromide. However, no induction of mutagenesis in the lacZ transgene could be detected in any tissue 14 days after single treatments of up to 50 mg/kg or after multiple treatments of as many as 10 daily treatments of 25 mg/kg MeBr. Copyright (C) 1998 Elsevier Science Ireland Ltd.
Subject
Nutrition
Methylating agents
N7-methylguanine
O6-Alkylguanine-DNA alkyltransferase
O6-Methylguanine
Pesticides
Somatic mutation
6 o alkylguanine DNA alkyltransferase
6 o methylguanine
7 methylguanine
Methyl bromide
Pesticide
Animal cell
Animal experiment
Bone marrow
Controlled study
DNA adduct
DNA methylation
Female
Gene mutation
Mouse
Nonhuman
Priority journal
Somatic mutation
Stomach
Tissue distribution
Transgenic mouse
Animals
DNA Adducts
DNA Methylation
Dose-Response Relationship, Drug
Guanine
Hydrocarbons, Brominated
Lac Operon
Male
Mice
Mice, Transgenic
Mutagenesis
Mutagens
O(6)-Methylguanine-DNA Methyltransferase
Rats
Rats, Sprague-Dawley
Mus musculus
To reference this document use:
http://resolver.tudelft.nl/uuid:eede6e70-a6fd-475b-b0f8-7910e7fa3c0b
DOI
https://doi.org/10.1016/s0304-3835(98)00262-6
TNO identifier
234821
ISSN
0304-3835
Source
Cancer Letters, 135 (1), 21-27
Document type
article