Print Email Facebook Twitter Soluble mannosylated myelin peptide inhibits the encephalitogenicity of autoreactive T cells during experimental autoimmune encephalomyelitis Title Soluble mannosylated myelin peptide inhibits the encephalitogenicity of autoreactive T cells during experimental autoimmune encephalomyelitis Author Kel, J. Oldenampsen, J. Luca, M. Drijfhout, J.W. Koning, F. Nagelkerken, L. TNO Kwaliteit van Leven Publication year 2007 Abstract We have previously shown that immunization with a mannosylated myelin peptide in complete adjuvant induces tolerance instead of disease in experimental autoimmune encephalomyelitis (EAE), a rodent model for multiple sclerosis. In this report we demonstrate that treatment with a soluble mannosylated epitope of proteolipid protein (M-PLP139-151) significantly inhibits disease mediated by autoreactive myelin-specific T cells during EAE. Treatment with M-PLP139-151, applied in different EAE models, significantly reduced the incidence of disease and the severity of clinical symptoms. Delayed-type hypersensitivity responses were abolished after peptide treatment, emphasizing the impact on peripheral T-cell reactivity. Histological analysis of spinal cord tissue from mice treated with M-PLP 139-151 revealed the presence of only few macrophages and T cells. Moreover, little expression of interferon-γ, interleukin-23, or major histocompatibility complex class II antigen was detected. Immune modulation by M-PLP139-151 was primarily antigen-specific because an irrelevant mannosylated peptide showed no significant effect on delayed-type hypersensitivity responses or on the course of EAE. Therefore, mannosylated antigens may represent a novel therapeutic approach for antigen-specific modulation of autoreactive T cells in vivo. Copyright © American Society for Investigative Pathology. Subject BiologyBiomedical Researchgamma interferoninterleukin 23major histocompatibility antigen class 2myelinproteolipidautoantigenepitopelymphocyte antigen receptormannosepeptide fragmentproteolipid proteinproteolipid protein 139 151proteolipid protein 139-151allergic encephalomyelitisanimal cellanimal experimentanimal modelanimal tissuearticlecontrolled studydrug mechanismfemalehistopathologyimmunizationimmunomodulationimmunoreactivityincidencemacrophagemousenonhumanpriority journalsolubilityspinal cordT lymphocyteanimalchemistrydrug effectimmunologyinflammationlymphocyte activationpathologyAnimalsAutoantigensEncephalomyelitis, Autoimmune, ExperimentalFemaleImmunodominant EpitopesInflammationLymphocyte ActivationMannoseMiceMyelin Proteolipid ProteinPeptide FragmentsReceptors, Antigen, T-CellSpinal CordT-Lymphocytes To reference this document use: http://resolver.tudelft.nl/uuid:ed5697c7-65fd-4572-ab0a-6683eca7293d DOI https://doi.org/10.2353/ajpath.2007.060335 TNO identifier 239795 ISSN 0002-9440 Source American Journal of Pathology, 170 (1), 272-280 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.