Identification of PLOD2 as Telopeptide Lysyl Hydroxylase, an Important Enzyme in Fibrosis
TNO Preventie en Gezondheid
van der Slot, A.J.
de Groot, J.
te Koppele, J.M.
The hallmark of fibrotic processes is an excessive accumulation of collagen. The deposited collagen shows an increase in pyridinoline cross-links, which are derived from hydroxylated lysine residues within the telopeptides. This change in cross-linking is related to irreversible accumulation of collagen in fibrotic tissues. The increase in pyridinoline cross-links is likely to be the result of increased activity of the enzyme responsible for the hydroxylation of the telopeptides (telopeptide lysyl hydroxylase, or TLH). Although the existence of TLH has been postulated, the gene encoding TLH has not been identified. By analyzing the genetic defect of Bruck syndrome, which is characterized by a pyridinoline deficiency in bone collagen, we found two missense mutations in exon 17 of PLOD2, thereby identifying PLOD2 as a putative TLH gene. Subsequently, we investigated fibroblasts derived from fibrotic skin of systemic sclerosis (SSc) patients and found that PLOD2 mRNA is highly increased indeed. Furthermore, increased pyridinoline cross-link levels were found in the matrix deposited by SSc fibroblasts, demonstrating a clear link between mRNA levels of the putative TLH gene (PLOD2) and the hydroxylation of lysine residues within the telopeptides. These data underscore the significance of PLOD2 in fibrotic processes. Chemicals/CAS: collagen, 9007-34-5; procollagen lysine 2 oxoglutarate 5 dioxygenase, 9059-25-0; Collagen, 9007-34-5; Cross-Linking Reagents; DNA, Complementary; Peptides; PLOD2 protein, human, EC 126.96.36.199; Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase, EC 188.8.131.52; RNA, Messenger
To reference this document use:
Procollagen lysine 2 oxoglutarate 5 dioxygenase
Telopeptide lysyl hydroxylase
Polymerase chain reaction
Amino Acid Sequence
Bone and Bones
DNA Mutational Analysis
Molecular Sequence Data
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase
Reverse Transcriptase Polymerase Chain Reaction
Journal of Biological Chemistry, 278 (278), 40967-40972