Title
Optimization of biomass, vitamins, and carotenoid yield on light energy in a flat-panel reactor using the A-stat technique
Author
Barbosa, M.J.
Zijffers, J.W.
Nisworo, A.
Vaes, W.
van Schoonhoven, J.
Wijffels, R.H.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
Acceleration-stat (A-stat) cultivations in which the dilution rate is continuously changed at a constant acceleration rate, leading to different average light intensities inside the photobioreactor, can supply more information and reduce experimental time compared with chemostat cultivations. The A-stat was used to optimize the biomass and product yield of continuous cultures of the microalgae D. tertiolecta in a flat-panel reactor. In this study, four different accelerations were studied, a pseudo steady state was maintained at acceleration rates of 0.00016 and 0.00029 h-2 and results were similar to those of the chemostat. An increase in the acceleration rate led to an increase in the deviation between results obtained in the A-stat and in the chemostats. We concluded that it is advantageous to use the A-stat instead of chemostats to determine culture characteristics and optimize a specific photobioreactor. The effect of average light intensity inside the photobioreactor on the production of vitamins C and E, lutein, and β-carotene was studied using the A-stat. The highest concentrations of these products were 3.48 ± 0.46, 0.33 ± 0.06, 5.65 ± 0.24, and 2.36 ± 0.38 mg g-1 respectively. These results were obtained at different average light intensities, showing the importance of optimizing each product on light intensity. © 2004 Wiley Periodicals, Inc.
Subject
Health
Analytical research
A-stat
Carotenoids
Flat-panel
Light
Microalgae
Vitamins
Algae
Biomass
Cell culture
Optimization
Vitamins
Carotenoid
Light intensities
Microalgae
Photobioreactors
Bioreactors
carotenoid
vitamin
alga
article
biomass
chemostat
light
light intensity
nonhuman
photobioreactor
reaction optimization
reactor
Algae, Green
Ascorbic Acid
Bioreactors
Carotenoids
Cell Culture Techniques
Cell Proliferation
Computer Simulation
Energy Transfer
Flow Injection Analysis
Gene Expression Regulation, Bacterial
Light
Models, Biological
Vitamin E
To reference this document use:
http://resolver.tudelft.nl/uuid:eaf0057d-599c-45ec-8849-f5b5a5135883
DOI
https://doi.org/10.1002/bit.20346
TNO identifier
238309
ISSN
0006-3592
Source
Biotechnology and Bioengineering, 89 (2), 233-242
Document type
article