Title
The effect of the food matrix on In Vivo immune responses to purified peanut allergens
Author
van Wijk, F.
Nierkens, S.
Hassing, I.
Feijen, M.
Koppelman, S.J.
de Jong, G.A.H.
Pieters, R.
Knippels, L.M.J.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
There is little knowledge about the factors that determine the allergenicity of food proteins. One aspect that remains to be elucidated is the effect of the food matrix on immune responses to food proteins. To study the intrinsic immunogenicity of allergens and the influence of the food matrix, purified peanut allergens (Ara h 1, Ara h 2, Ara h 3 or Ara h 6) and a whole peanut extract (PE) were tested in the popliteal lymph node assay (PLNA) and in an oral model of peanut hypersensitivity. In the PLNA, peanut proteins were injected into the hind footpad of BALB/ c mice; in the oral exposure experiments C3H/HeOuJ mice were gavaged weekly with PE or allergens in the presence of cholera toxin (CT). Upon footpad injection, none of the allergens induced significant immune activation. In contrast, PE induced an increase in cell number, cytokine production, and activation of antigen-presenting cells. Furthermore, the presence of a food matrix enhanced the immune response to the individual allergens. Oral exposure to the purified allergens in the presence of CT induced specific IgE responses, irrespective of the presence of a food matrix. These results suggest that purified peanut allergens possess little intrinsic immune-stimulating capacity in contrast to a whole PE. Moreover, the data indicate that the food matrix can influence responses to individual proteins and, therefore, the food matrix must be taken into account when developing models for allergenic potential assessment. © The Author 2005. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.
Subject
Nutrition Toxicology
Food technology
Food matrix
Immunogenicity
Mouse model
Peanut allergens
allergen
cholera toxin
cytokine
immunoglobulin E
allergenicity
animal cell
animal experiment
antigen presenting cell
antigen purification
article
assay
cell count
controlled study
cytokine production
feeding
female
food allergy
foot pad
immune response
immunogenicity
immunoreactivity
immunostimulation
in vivo study
lymph node
mouse
mouse strain
nonhuman
peanut
Allergens
Animals
Antigens, CD
Antigens, CD80
Antigens, CD86
Arachis hypogaea
Cholera Toxin
Cytokines
Dietary Fats
Female
Glycoproteins
Immunoglobulin E
Immunoglobulin G
Intercellular Adhesion Molecule-1
Lymph Nodes
Membrane Glycoproteins
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Peanut Hypersensitivity
Plant Proteins
Ara
Arachis hypogaea
To reference this document use:
http://resolver.tudelft.nl/uuid:e9f8ad85-9837-4853-8041-71b704fde14f
DOI
https://doi.org/10.1093/toxsci/kfi187
TNO identifier
238619
ISSN
1096-6080
Source
Toxicological Sciences, 86 (2), 333-341
Document type
article