Print Email Facebook Twitter The effect of the food matrix on In Vivo immune responses to purified peanut allergens Title The effect of the food matrix on In Vivo immune responses to purified peanut allergens Author van Wijk, F. Nierkens, S. Hassing, I. Feijen, M. Koppelman, S.J. de Jong, G.A.H. Pieters, R. Knippels, L.M.J. TNO Kwaliteit van Leven Publication year 2005 Abstract There is little knowledge about the factors that determine the allergenicity of food proteins. One aspect that remains to be elucidated is the effect of the food matrix on immune responses to food proteins. To study the intrinsic immunogenicity of allergens and the influence of the food matrix, purified peanut allergens (Ara h 1, Ara h 2, Ara h 3 or Ara h 6) and a whole peanut extract (PE) were tested in the popliteal lymph node assay (PLNA) and in an oral model of peanut hypersensitivity. In the PLNA, peanut proteins were injected into the hind footpad of BALB/ c mice; in the oral exposure experiments C3H/HeOuJ mice were gavaged weekly with PE or allergens in the presence of cholera toxin (CT). Upon footpad injection, none of the allergens induced significant immune activation. In contrast, PE induced an increase in cell number, cytokine production, and activation of antigen-presenting cells. Furthermore, the presence of a food matrix enhanced the immune response to the individual allergens. Oral exposure to the purified allergens in the presence of CT induced specific IgE responses, irrespective of the presence of a food matrix. These results suggest that purified peanut allergens possess little intrinsic immune-stimulating capacity in contrast to a whole PE. Moreover, the data indicate that the food matrix can influence responses to individual proteins and, therefore, the food matrix must be taken into account when developing models for allergenic potential assessment. © The Author 2005. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. Subject Nutrition ToxicologyFood technologyFood matrixImmunogenicityMouse modelPeanut allergensallergencholera toxincytokineimmunoglobulin Eallergenicityanimal cellanimal experimentantigen presenting cellantigen purificationarticleassaycell countcontrolled studycytokine productionfeedingfemalefood allergyfoot padimmune responseimmunogenicityimmunoreactivityimmunostimulationin vivo studylymph nodemousemouse strainnonhumanpeanutAllergensAnimalsAntigens, CDAntigens, CD80Antigens, CD86Arachis hypogaeaCholera ToxinCytokinesDietary FatsFemaleGlycoproteinsImmunoglobulin EImmunoglobulin GIntercellular Adhesion Molecule-1Lymph NodesMembrane GlycoproteinsMiceMice, Inbred BALB CMice, Inbred C3HPeanut HypersensitivityPlant ProteinsAraArachis hypogaea To reference this document use: http://resolver.tudelft.nl/uuid:e9f8ad85-9837-4853-8041-71b704fde14f DOI https://doi.org/10.1093/toxsci/kfi187 TNO identifier 238619 ISSN 1096-6080 Source Toxicological Sciences, 86 (2), 333-341 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.