Title
Application of liquid chromatography-mass spectrometry to measure the concentrations and study the synthesis of short chain fatty acids following stable isotope infusions
Author
Meesters, R.J.W.
van Eijk, H.M.H.
ten Have, G.A.M.
de Graaf, A.A.
Venema, K.
van Rossum, B.E.J.
Deutz, N.E.P.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
A new method involving zinc sulphate deproteinization was developed to study short chain fatty acids (SCFA) production in the colon and subsequent occurrence of SCFA in blood. SCFA were baseline separated in a 30 min cycle using ion-exclusion chromatography and detected by mass spectrometry. Concentrations could be measured down to 10 μM and isotopomeric distributions could be assessed, enabling the conduction of tracer studies to study changes in SCFA synthesis. The applicability of the method was tested in an extensively characterized pig model yielding portal SCFA concentrations ranging from 70 μM (butyric acid) to 150 μM (propionic acid) to 440 μM (acetic acid) prior to butyrate tracer infusion, reaching butyric acid isotopic steady state within 2 h. © 2007 Elsevier B.V. All rights reserved.
Subject
Health
Biomedical Research
LC-MS
SCFA
Short chain fatty acids
Stable isotope
Concentration (process)
Measurement theory
Synthesis (chemical)
Short chain fatty acids
Stable isotope
Liquid chromatography
acetic acid
butyric acid
propionic acid
short chain fatty acid
tracer
animal experiment
colon
fatty acid synthesis
ion exclusion chromatography
mass spectrometry
nonhuman
priority journal
steady state
Animals
Chromatography, Gel
Fatty Acids
Isotopes
Mass Spectrometry
Swine
Suidae
To reference this document use:
http://resolver.tudelft.nl/uuid:e84b06eb-62e5-4c6c-9f4f-aa43e1cb4cc8
DOI
https://doi.org/10.1016/j.jchromb.2007.03.044
TNO identifier
240040
ISSN
1570-0232
Source
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 854 (1-2), 57-62
Document type
article