Title
Identification of disease- and nutrient-related metabolic fingerprints in osteoarthritic guinea pigs
Author
Lamers, R.-J.A.N.
de Groot, J.
Spies-Faber, E.J.
Jellema, R.H.
Kraus, V.B.
Verzijl, N.
te Koppele, J.M.
Spijksma, G.K.
Vogels, J.T.W.E.
van der Greef, J.
van Nesselrooij, J.H.J.
Publication year
2003
Abstract
Osteoarthritis (OA), one of the most common diseases among the elderly, is characterized by the progressive destruction of joint tissues. Its etiology is largely unclear and no effective disease-modifying treatment is currently available. Metabolic fingerprinting provides a novel tool for the identification of biomarkers. A metabolic fingerprint consists of a typical combination of metabolites in a biological fluid and is identified by a combination of 1H NMR spectroscopy and multivariate data analysis (MVDA). The current feasibility study was aimed at identifying a metabolic fingerprint for OA and applying this in a nutritional intervention study. Urine samples were collected from osteoarthritic male Hartley guinea pigs (n = 44) at 10 and 12 mo of age, treated from 4 mo onward with variable vitamin C doses (2.5-3, 30 and 150 mg/d) and from healthy male Strain 13 guinea pigs (n = 8) at 12 mo of age, treated with 30 mg vitamin C/d. NMR measurements were performed on all urine samples. Subsequently, MVDA was carried out on the data obtained using NMR. An NMR fingerprint was identified that reflected the osteoarthritic changes in guinea pigs. The metabolites that comprised the fingerprint indicate that energy and purine metabolism are of major importance in OA. Metabolic fingerprinting also allowed detection of differences in OA-specific metabolites induced by different dietary vitamin C intakes. This study demonstrates the feasibility of metabolic fingerprinting to identify disease-specific profiles of urinary metabolites. NMR fingerprinting is a promising means of identifying new disease markers and of gaining fresh insights into the pathophysiology of disease.
Subject
Biology
Analytical research
Biomedical research
1H NMR spectroscopy
Metabolic fingerprinting
Multivariate data analysis
Osteoarthritis
Vitamin C
ascorbic acid
biological marker
purine
animal experiment
animal model
article
controlled study
data analysis
dietary intake
disease marker
energy metabolism
guinea pig
inbred strain
male
metabolic fingerprint
metabolism
metabolite
multivariate analysis
nonhuman
nutrient
nutrition
osteoarthritis
pathophysiology
proton nuclear magnetic resonance
purine metabolism
strain difference
urinalysis
vitamin intake
Animal Nutrition Physiology
Animals
Ascorbic Acid
Diet
Dose-Response Relationship, Drug
Energy Metabolism
Guinea Pigs
Magnetic Resonance Spectroscopy
Male
Multivariate Analysis
Osteoarthritis
Peptide Mapping
Purines
Treatment Outcome
Animalia
Cavia
Cavia porcellus
Sus scrofa
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http://resolver.tudelft.nl/uuid:e689cd7c-6abd-4d05-9ea4-91c3d7c2d832
TNO identifier
237136
ISSN
0022-3166
Source
Journal of Nutrition, 133 (6), 1776-1780
Document type
article