Title
Molecular characterization of two lipoxygenases from barley
Author
van Mechelen, J.R.
Schuurink, R.C.
Smits, M.
Graner, A.
Douma, A.C.
Sedee, N.J.A.
Schmitt, N.F.
Valk, B.E.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and LoxB map on chromosome 4 and LoxC on chromosome 7. Two isoenzymes, LOX1 and LOX2, have been purified previously from germinating barley and characterized. LOX1 is encoded by LoxA, while LOX2 is encoded by LoxC. The product related to the third cDNA (loxB) has not been identified so far, suggesting a low protein abundance for the corresponding isoform in barley. Transcripts corresponding with these LOX genes are predominantly observed in grain and in seedling, whereas transcripts corresponding to LoxB and LoxC are also observed in mature vegetative tissue. No lipoxygenase mRNA could be detected in aleurone layer of germinating grain. No significant differences in lipoxygenase mRNA levels were observed in developing grains grown under dormant or non-dormant conditions, suggesting that LOX is not directly involved in induction of grain dormancy. Chemicals/CAS: Antibodies, Monoclonal; Isoenzymes; Lipoxygenase, EC 1.13.11.12; Recombinant Proteins; RNA, Messenger
Subject
Barley
Chromosomal mapping
Grain dormancy
Hordeum distichum
Jasmonate (jasmonic acid)
Lipoxygenase (LOX)
Chromosomal localization
Complementary DNA
Dormancy
Gene locus
Germination
Lipoxygenase
Messenger RNA
Restriction fragment length polymorphism
Sequence homology
Southern blotting
Amino Acid Sequence
Antibodies, Monoclonal
Base Sequence
Chromosomes
Cloning, Molecular
Gene Dosage
Gene Expression
Genes, Plant
Germination
Hordeum
Isoenzymes
Lipoxygenase
Molecular Sequence Data
Physical Chromosome Mapping
Polymorphism, Restriction Fragment Length
Recombinant Proteins
RNA, Messenger
Sequence Analysis
Sequence Homology, Amino Acid
Embryophyta
Hordeum
Hordeum vulgare subsp. vulgare
Loxa
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http://resolver.tudelft.nl/uuid:e628a32a-6ba3-4180-9f09-2063d82fc07f
DOI
https://doi.org/10.1023/a:1006118003998
TNO identifier
71511
ISSN
0167-4412
Source
Plant Molecular Biology, 39 (6), 1283-1298
Document type
article