Print Email Facebook Twitter Glucose detection at bare and sputtered platinum electrodes coated with polypyrrole and glucose oxidase Title Glucose detection at bare and sputtered platinum electrodes coated with polypyrrole and glucose oxidase Author van Os, P.J.H.J. Bult, A. Koopal, C.G.J. van Bennekom, W.P. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1996 Abstract Recently a glucose sensor has been developed, which is easily prepared, has a good stability and high glucose response, and is interference free for ascorbic acid. The sensor was made by galvanostatic polymerization of 0.3 M pyrrole in a HEPES buffer pH 7.0 with 200 U ml-1 glucose oxidase (GOx) using a current of 1 μA. In this study, bare and sputtered platinum electrodes, coated with polypyrrole in the presence of GOx and/or flavine adenine dinucleotide (FAD), are compared. FAD in excess in the polymerization or sample solution did not increase the glucose response. The selectivity with respect to other mono and oligosaccharides was good. The mechanism of electron transfer was studied. From measurements at low potentials and at anaerobic conditions there is a strong indication that the glucose sensor is a combination of indirect and direct electron-transfer (first and third generation, respectively). Chemicals/CAS: glucose oxidase, 9001-37-0; platinum, 7440-06-4; polypyrrole, 30604-81-0 Subject (Direct) electron-transfer(Poly-)pyrrole(Spudered)platinumFADFlavine adenine dinucIeotideGlucose oxidaseGOxMultichannel electrochemical detectionSelectivity To reference this document use: http://resolver.tudelft.nl/uuid:e5db0cde-ac46-44ec-9d87-6167658d6a3f DOI https://doi.org/10.1016/s0003-2670(96)00327-3 TNO identifier 233716 ISSN 0003-2670 Source Analytica Chimica Acta, 335 (3), 209-216 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.