Print Email Facebook Twitter Skin and urine pentosidine weakly correlate with joint damage in a cohort of patients with early signs of osteoarthritis (CHECK) Title Skin and urine pentosidine weakly correlate with joint damage in a cohort of patients with early signs of osteoarthritis (CHECK) Author Vos, P.A.J.M. de Groot, J. Huisman, A.M. Oostveen, J.C.M. Marijnissen, A.C.A. Bijlsma, J.W.J. van El, B. Zuurmond, A.M. Lafeber, F.P.J.G. TNO Kwaliteit van Leven Publication year 2010 Abstract Objectives: Age-related changes in articular cartilage are likely to play a role in the aetiology of osteoarthritis (OA). One of the major age-related changes in cartilage is the accumulation of advanced-glycation-endproducts (AGEs). Since, cartilage tissue is not readily available from patients for studying AGE levels, alternative approaches such as analyzing skin and urine are needed to study the role of cartilage AGE levels in OA. Methods: Paired human skin and cartilage samples were obtained post mortem. Paired skin and urine samples were obtained from the CHECK cohort (early OA patients). Pentosidine levels were measured by high-performance liquid chromatography (HPLC). As marker of cumulative cartilage damage X-rays of both knees and hips were scored. Urinary CTXII (uCTXII) levels were measured, to assess current cartilage breakdown. Results: Cartilage and skin pentosidine correlate well (R= 0.473, P= 0.05). Skin pentosidine was higher in mild (summed (Kellgren & Lawrence K&L) over four large joints ≥4) compared to no (summed K&L≤3) radiographic OA (P= 0.007). Urinary pentosidine was not different between these two groups. Skin pentosidine levels were not related to cartilage breakdown (highest vs lowest tertile of uCTXII). Urinary pentosidine, however, was higher in the highest compared to the lowest uCTXII tertile (P= 0.009). Multiple regression analysis showed age to be the only predictor of the summed K&L score and age, creatinine clearance and urinary pentosidine as predictors of uCTXII. Conclusion: The higher skin and urinary pentosidine levels in those with mild compared to no radiographic joint damage and low vs high cartilage breakdown respectively suggest that AGEs may contribute to disease susceptibility and/or progression. However, relations are weak and cannot be used as surrogate markers of severity of OA. © 2010 Osteoarthritis Research Society International. Subject BiologyBiomedical ResearchAgeCanineCohortOsteoarthritisPentosidinepentosidineadultagedarticlearticular cartilageautopsycohort analysiscontrolled studycreatinine clearancedisease severityfemalehigh performance liquid chromatographyhiphip osteoarthritishumanjoint injurykneeknee osteoarthritismajor clinical studymalemultiple regressionpredictor variablepriority journalradiographyskinurinalysisX ray To reference this document use: http://resolver.tudelft.nl/uuid:e5bf2e9a-a5a6-4c93-8273-80883688d2b7 TNO identifier 409808 ISSN 1063-4584 Source Osteoarthritis and Cartilage, 18 (10), 1329-1336 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.