Title
Analysis of patulin in dutch food, an evaluation of a SPE based method
Author
Boonzaaijer, G.
Bobeldijk, I.
van Osenbruggen, W.A.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
A reliable method for the determination of patulin in apple juice, apple puree and apples was developed using two clean-up steps and high performance liquid chromatography and diode array (HPLC-DAD) analysis. Baseline separation from hydroxymethyl furfural (HMF) was obtained. The performance characteristics of the method were determined at four concentration levels in two matrices and fulfill the requirements of the European Commission (EC) for this type of samples. The level of quantification was set to 25 μg/l or 25 μg/kg, the RSD for repeatability at this level is 11% for apple juice and 17% for apples, the recovery is 87% for apple juice and 63% for apples. The reproducibility was determined in apples and is 11% (at a level of 200 μg/kg). The method was used for determination of patulin in 63 commercial apple products available on the Dutch market. With the exception of one sample, the level of patulin was lower than the detection limit in all the samples. No statistically significant difference was found between conventional and organic samples. © 2004 Elsevier Ltd. All rights reserved.
Subject
Nutrition
Analytical research
Apples
Diode array
Liquid chromatography
Patulin
Solid phase extraction
5 hydroxymethylfurfural
organic compound
patulin
analytic method
apple
apple juice
article
commercial phenomena
concentration (parameters)
controlled study
diode
European Union
food analysis
food contamination
food control
food quality
high performance liquid chromatography
marketing
Netherlands
reliability
reproducibility
separation technique
solid phase extraction
statistical significance
tomato
Malus x domestica
To reference this document use:
http://resolver.tudelft.nl/uuid:e4876b64-429e-4ada-b2a1-f1ecb4a4ed52
DOI
https://doi.org/10.1016/j.foodcont.2004.06.020
TNO identifier
238675
ISSN
0956-7135
Source
Food Control, 16 (7), 587-591
Document type
article