Title
Effect of apolipoprotein E and insulin resistance on VLDL particles in combined hyperlipidemic patients
Author
Sijbrands, E.J.G.
Westendorp, R.G.J.
Hoffer, M.J.V.
Frants, R.R.
Meinders, A.E.
Souverijn, J.H.M.
Leuven, J.A.G.
van der Laarse, A.
Havekes, L.M.
Smelt, A.H.M.
Gaubius Instituut TNO
Publication year
1996
Abstract
Apolipoprotein (ape) E2 and high insulin levels are associated with the severity of hypertriglyceridemia in patients with combined hyperlipidemia. To study how these determinants affect very low-density lipoprotein (VLDL) in combined hyperlipidemic patients, we characterized VLDL particles in 106 unrelated patients with combined hyperlipidemia. The study was performed after 9 weeks of standardized dietary intake and after an overnight fast. Patients heterozygous for apoE2 had significantly higher mean levels of VLDL cholesterol by 0.71 mmol/l (95% CI, 0.30 to 1.12 mmol/l, P < 0.005) and VLDL triglycerides by 0.88 mmol/l (95% CI, 0.30 to 1.47 mmol/l, P < 0.005) compared to patients without apoE2. The VLDL triglyceride content per particle and the calculated diameter of the VLDL particles were similar in both groups, which indicate a higher number of circulating VLDL particles in heterozygous apoE2 carriers. Patients with high fasting insulin levels (≤ 80 pmol/l) had a higher mean serum VLDL triglyceride level by 0.56 mmol/l (95% CI, 0.04 to 1.07 mmol/l, P < 0.05). The calculated VLDL diameter was larger by 3.7 nm (95% CI, 1.2 to 6.2 nm, P < 0.005) and the particles contained more triglycerides by 2.7 weight percent (95% CI, 0.3 to 5.1 weight percent, P < 0.05). These insulin-dependent changes in VLDL particles were only present in the absence of apoE2. In conclusion, patients heterozygous for apoE2 have higher numbers of circulating VLDL particles, whereas patients with high fasting insulin levels have larger, triglyceride enriched VLDL particles. Chemicals/CAS: Apolipoproteins E; Cholesterol, VLDL; Insulin, 11061-68-0; Lipoproteins, VLDL; Triglycerides; very low density lipoprotein triglyceride
Subject
Biology
ApoE
Combined hyperlipidemia
Hyperinsulinemia
Particle size
VLDL
apolipoprotein e
insulin
very low density lipoprotein
adult
aged
article
clinical trial
controlled clinical trial
controlled study
diet
female
genetic analysis
heterozygosity
human
human tissue
hyperlipidemia
hypertriglyceridemia
insulin resistance
lipid analysis
major clinical study
male
priority journal
Adult
Aged
Apolipoproteins E
Cholesterol, VLDL
Fasting
Female
Humans
Hyperlipidemia, Familial Combined
Insulin
Insulin Resistance
Lipoproteins, VLDL
Male
Middle Aged
Particle Size
Triglycerides
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DOI
https://doi.org/10.1016/0021-9150(96)05901-1
TNO identifier
233503
ISSN
0021-9150
Source
Atherosclerosis, 126 (2), 197-205
Document type
article