Title
Intercellular communication and cell proliferation in precision-cut rat liver slices: Effect of medium composition and DDT
Author
Graaf, I.A.M.
Tajima, O.
Groten, J.P.
Wolterbeek, A.P.M.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2000
Abstract
Gap junctional intercellular communication (GJIC) and cell proliferation were studied in control and 1,1'-bis(p-chlorophenyl)-2,2,2,-trichloroethane (DDT) treated precision-cut liver slices of rat by evaluating connexin 32 (Cx32) expression and 5-bromo-2'-deoxyuridine (BrdU) incorporation. In addition, the effect of different culture media (RPMI and WME) on control and DDT influenced Cx32 expression and cell proliferation was determined. Cx32 expression in control precision-cut liver slices was maintained during 8 h of culturing, but decreased after prolonged culturing. Control cell proliferation was significantly higher when WME was used as culture medium than when RPMI was used. In slices treated with DDT Cx32 expression was decreased. In slices cultured in RPMI medium, this decrease preceded a dose-dependent increase in cell proliferation. These results show the usefulness of precision-cut liver slices in studying cellular proliferation and intercellular communication. Copyright (C) 2000 Published by Elsevier Science Ireland Ltd.
Subject
Nutrition
1,1'-Bis(p-chlorophenyl)-2,2,2,-trichloroethane
3-Aminopropyltriethoxysilane
5-Bromo-2'-deoxyuridine
Cell proliferation
Connexin 32
Diaminebenzidine
Dimethyl sulfoxide
Fetal bovine serum
Gap junctional intercellular communication
Histomorphology
Intercellular communication
Lactate dehydrogenase
Phosphate-buffered saline
Precision-cut liver slices
RPMI 1640 medium
Williams medium E
Broxuridine
Chlorphenotane
Connexin 32
Dimethyl sulfoxide
Lactate dehydrogenase
Tissue culture medium
Animal experiment
Animal model
Animal tissue
Carcinogenesis
Cell communication
Cell proliferation
Controlled study
Gap junction
Immunocytochemistry
Liver slice
Male
Morphometrics
Nonhuman
Priority journal
Rat
Animals
Blotting, Western
Bromodeoxyuridine
Cell Division
Cells, Cultured
Connexins
Culture Media
DDT
Dose-Response Relationship, Drug
Gap Junctions
Immunohistochemistry
L-Lactate Dehydrogenase
Liver
Male
Rats
Rats, Wistar
Signal Transduction
Time Factors
Bovinae
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http://resolver.tudelft.nl/uuid:e2c47453-41a8-4edf-9e14-dc3581144501
DOI
https://doi.org/10.1016/s0304-3835(00)00377-3
TNO identifier
87150
ISSN
0304-3835
Source
Cancer Letters, 154 (1), 53-62
Bibliographical note
Correspondence Address: Wolterbeek, A.P.M.; TNO Voeding, PO Box 360, 3700 AJ Zeist, Netherlands; email: wolterbeek@voeding.tno.nl Chemicals/CAS: Bromodeoxyuridine, 59-14-3; connexin 32; Connexins; Culture Media; DDT, 50-29-3; L-Lactate Dehydrogenase, EC 1.1.1.27
Document type
article