90-Day feeding and genotoxicity studies on a refined arachidonic acid-rich oil

90-Day feeding and genotoxicity studies on a refined arachidonic acid-rich oil

Casterton, P.L.
Curry, L.L.
Lina, B.A.R.
Wolterbeek, A.P.M.
Kruger, C.L.
TNO Kwaliteit van Leven

2009

The safety of a refined arachidonic acid-rich oil (RAO) was evaluated for reverse mutation, chromosome aberration and gene mutation, and in a 90-day Wistar rat feeding study with in utero exposure. The results of the genotoxicity assays were all negative. The in utero phase of the 90-day study involved dietary exposure to 0.5%, 1.5% and 5% RAO and two controls diets, a standard feed low-fat diet and a high-fat diet supplemented with 5% corn oil. This exposure covered four-weeks prior to mating, through mating, gestation and lactation until offspring (F1) weaning. A subsequent 90-day feeding study in the F1 rats evaluated the same test and control diets. Statistically significant effects were seen for selected histopathology, clinical chemistry and organ weight endpoints; however, other than increased absolute and relative monocytes seen in both sexes of high-dose rats, the observations were not attributed to treatment for one or more reasons. Based on these findings, no adverse treatment-related effects for RAO were seen at up to 5% in the diet, equivalent to an overall average RAO intake of 3170 mg/kg bwt/day. These and similar findings for other refined ARA-rich oils establish a strong body of evidence for the safety of this RAO. © 2009 Elsevier Ltd. All rights reserved.

Nutrition
Toxicology and Applied Pharmacology
90-Day feeding study
ARA-rich oil
Arachidonic acid
Genotoxicity

http://resolver.tudelft.nl/uuid:e2284dd3-967b-406a-9d80-6cb07aea984c

https://doi.org/10.1016/j.fct.2009.06.036

241780

0278-6915

Food and Chemical Toxicology, 47 (10), 2407-2418

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